Assessment of survival during starvation of Escherichia coli and Klebsiella pneumoniae in artificial urine: analysis of the kinetics of colony formation

O. Julia, J. Vives-Rego, M. Vilamú, R. López-Amorós, F. Utzet

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Abstract

A kinetic model of colony formation was proposed by Hattori, based on a count of the colonies that appear on a plate in successive short intervals of time. In this model, three parameters (λ, tr and N∞) are defined, which reflect the ability of a bacterium to yield colonies and allow us to described the dynamics of bacterial populations in soil and of E. coli at different growth phases. In this paper we report a reparametrization of the kinetic model of colony formation, with the aim of facilitating more accurate calculation of λ and tr. Moreover, we observed that during the starvation of E. coli and K. pneumoniae in urine, λ can be used to assess survival, since this parameter clearly decreases during starvation. Retardation time values (tr) were similar in E. coli and K. pneumoniae throughout the starvation experimental period. © 1995 Kluwer Academic Publishers.
Original languageEnglish
Pages (from-to)371-375
JournalAntonie van Leeuwenhoek
Volume67
Issue number4
DOIs
Publication statusPublished - 1 Dec 1995

Keywords

  • colony formation
  • first order reaction kinetics
  • stravation
  • survival
  • urine

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