Assessment of a platelet function analyser in horses: Reference range and influence of a platelet aggregation inhibitor

Didac Segura, L. Monreal, Y. Espada, J. Pastor, I. Mayós, J. Homedes

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23 Citations (Scopus)

Abstract

The objective of this study was to assess whether a new human platelet function analyser (the PFA-100) could be used to evaluate platelet function in horses and detect acetylsalicylic acid (ASA)-induced platelet dysfunctions. Citrated blood samples from 40 healthy horses were processed to obtain reference values for closure time (CT) using cartridges with collagen-ADP (CT-ADP) and collagen-epinephrine (CT-EPI) as platelet agonists. In addition, CT-ADP and CT-EPI were also measured before and 24 h after oral ASA administration in another 12 healthy horses. The sensitivity and specificity of the test were also determined. In normal horses, means±SD value for CT-ADP was 85.1±13.1 s (median, 82 s), and CT-EPI ranged from 158 to >300 s (median 291 s). Calculated reference ranges were 60.5-115.9 s and 158.5->300 s for CT-ADP and CT-EPI, respectively. Administration of ASA significantly (P<0.001) prolonged CT-ADP values from 91.0±13 to 113.5±14.4 s, and CT-EPI values were also significantly (P<0.008) prolonged after ASA administration. Sensitivity and specificity results for ADP cartridges showed that a prolonged CT value would be highly suggestive of a platelet aggregation inhibition. In conclusion, ADP cartridges can be used in horses to assess primary haemostasis and may be a valuable test for the detection of platelet aggregation inhibition. © 2004 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)108-112
JournalVeterinary Journal
Volume170
DOIs
Publication statusPublished - 1 Jul 2005

Keywords

  • Acetylsalicylic acid
  • Horse
  • Platelet function
  • Platelet function analyser
  • Primary haemostasis

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