Assessment in vitro of cytogenetic and genotoxic effects of propolis on human lymphocytes

A. Montoro; J. M. Soriano; J. F. Barquinero; M. Almonacid; A. Montoro; G. Verdú; V. Sahuquillo; J. I. Villaescusa; N. Sebastià

doi:https://doi.org/10.1016/j.fct.2011.10.053

Assessment in vitro of cytogenetic and genotoxic effects of propolis on human lymphocytes

A. Montoro, J. M. Soriano, J. F. Barquinero, M. Almonacid, A. Montoro, G. Verdú, V. Sahuquillo, J. I. Villaescusa, N. Sebastià

Research output: Contribution to journal › Article › Research › peer-review

13 Citations (Scopus)

Abstract

We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocytes which were exposed to increasing concentrations (0-2000μgml -1). The results indicated that EEP reduced significantly the mitotic index (MI) and proliferation index (PI) when high concentrations of EEP were used. Sister chromatid exchange (SCE) rates indicated that EEP could have genotoxic effects at high concentrations. Exposure of the cells to the amount of ethanol used as solvent did not alter either the MI and cell proliferation kinetics (CPK), or the rate of SCE. The results showed: (a) statistical increase in the percentage the cells with CAs and in the frequency of SCE at the highest concentrations, (b) a decrease in MI and in the CPK values was observed, (c) no effect was noticed in negative controls. In conclusion, it can be assumed that high concentrations of EEP have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes. © 2011 Elsevier Ltd.

Original language	English
Pages (from-to)	216-221
Journal	Food and Chemical Toxicology
Volume	50
DOIs	https://doi.org/10.1016/j.fct.2011.10.053
Publication status	Published - 1 Feb 2012

Keywords

Cytotoxicity
Ethanolic extract of propolis
Genotoxicity
Human lymphocytes
In vitro

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https://doi.org/10.1016/j.fct.2011.10.053

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title = "Assessment in vitro of cytogenetic and genotoxic effects of propolis on human lymphocytes",

abstract = "We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocytes which were exposed to increasing concentrations (0-2000μgml -1). The results indicated that EEP reduced significantly the mitotic index (MI) and proliferation index (PI) when high concentrations of EEP were used. Sister chromatid exchange (SCE) rates indicated that EEP could have genotoxic effects at high concentrations. Exposure of the cells to the amount of ethanol used as solvent did not alter either the MI and cell proliferation kinetics (CPK), or the rate of SCE. The results showed: (a) statistical increase in the percentage the cells with CAs and in the frequency of SCE at the highest concentrations, (b) a decrease in MI and in the CPK values was observed, (c) no effect was noticed in negative controls. In conclusion, it can be assumed that high concentrations of EEP have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes. {\textcopyright} 2011 Elsevier Ltd.",

keywords = "Cytotoxicity, Ethanolic extract of propolis, Genotoxicity, Human lymphocytes, In vitro",

author = "A. Montoro and Soriano, {J. M.} and Barquinero, {J. F.} and M. Almonacid and A. Montoro and G. Verd{\'u} and V. Sahuquillo and Villaescusa, {J. I.} and N. Sebasti{\`a}",

year = "2012",

month = feb,

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doi = "https://doi.org/10.1016/j.fct.2011.10.053",

language = "English",

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pages = "216--221",

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T1 - Assessment in vitro of cytogenetic and genotoxic effects of propolis on human lymphocytes

AU - Montoro, A.

AU - Soriano, J. M.

AU - Barquinero, J. F.

AU - Almonacid, M.

AU - Montoro, A.

AU - Verdú, G.

AU - Sahuquillo, V.

AU - Villaescusa, J. I.

AU - Sebastià, N.

PY - 2012/2/1

Y1 - 2012/2/1

N2 - We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocytes which were exposed to increasing concentrations (0-2000μgml -1). The results indicated that EEP reduced significantly the mitotic index (MI) and proliferation index (PI) when high concentrations of EEP were used. Sister chromatid exchange (SCE) rates indicated that EEP could have genotoxic effects at high concentrations. Exposure of the cells to the amount of ethanol used as solvent did not alter either the MI and cell proliferation kinetics (CPK), or the rate of SCE. The results showed: (a) statistical increase in the percentage the cells with CAs and in the frequency of SCE at the highest concentrations, (b) a decrease in MI and in the CPK values was observed, (c) no effect was noticed in negative controls. In conclusion, it can be assumed that high concentrations of EEP have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes. © 2011 Elsevier Ltd.

AB - We evaluated the genetic damage by ethanolic extract of propolis (EEP) induced to human lymphocytes which were exposed to increasing concentrations (0-2000μgml -1). The results indicated that EEP reduced significantly the mitotic index (MI) and proliferation index (PI) when high concentrations of EEP were used. Sister chromatid exchange (SCE) rates indicated that EEP could have genotoxic effects at high concentrations. Exposure of the cells to the amount of ethanol used as solvent did not alter either the MI and cell proliferation kinetics (CPK), or the rate of SCE. The results showed: (a) statistical increase in the percentage the cells with CAs and in the frequency of SCE at the highest concentrations, (b) a decrease in MI and in the CPK values was observed, (c) no effect was noticed in negative controls. In conclusion, it can be assumed that high concentrations of EEP have a cyto and genotoxic effect, in vitro, for human peripheral lymphocytes. © 2011 Elsevier Ltd.

KW - Cytotoxicity

KW - Ethanolic extract of propolis

KW - Genotoxicity

KW - Human lymphocytes

KW - In vitro

U2 - https://doi.org/10.1016/j.fct.2011.10.053

DO - https://doi.org/10.1016/j.fct.2011.10.053

M3 - Article

SN - 0278-6915

VL - 50

SP - 216

EP - 221

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

ER -

Assessment in vitro of cytogenetic and genotoxic effects of propolis on human lymphocytes

Abstract

Keywords

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