Assay of β-galactosidase activity by Flow Injection Analysis (FIA)

J. Cairó; M. Vidal; A. Villaverde; F. Valero; F. J. Lafuente; C. Solà

doi:10.1007/BF00185020

Assay of β-galactosidase activity by Flow Injection Analysis (FIA)

J. Cairó, M. Vidal, A. Villaverde, F. Valero, F. J. Lafuente, C. Solà

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

A novel method to analyze β-galactosidase by Flow Injection Analysis is presented with a linear working range extended to at least 2150 U/mL, being the detection limit 25 U/mL with 55 samples per hour frecuency and a BSD of 0.954% versus 2.4% obtained by manual assay. The method was tested with optimal results with samples from Escherichia coli cultures producing β-galactosidase. © 1991 Chapman & Hall.

Original language	English
Pages (from-to)	389-392
Journal	Biotechnology Techniques
Volume	5
Issue number	5
DOIs	https://doi.org/10.1007/BF00185020
Publication status	Published - 1 Sept 1991

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title = "Assay of β-galactosidase activity by Flow Injection Analysis (FIA)",

abstract = "A novel method to analyze β-galactosidase by Flow Injection Analysis is presented with a linear working range extended to at least 2150 U/mL, being the detection limit 25 U/mL with 55 samples per hour frecuency and a BSD of 0.954% versus 2.4% obtained by manual assay. The method was tested with optimal results with samples from Escherichia coli cultures producing β-galactosidase. {\textcopyright} 1991 Chapman & Hall.",

author = "J. Cair{\'o} and M. Vidal and A. Villaverde and F. Valero and Lafuente, {F. J.} and C. Sol{\`a}",

year = "1991",

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doi = "10.1007/BF00185020",

language = "English",

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T1 - Assay of β-galactosidase activity by Flow Injection Analysis (FIA)

AU - Cairó, J.

AU - Vidal, M.

AU - Villaverde, A.

AU - Valero, F.

AU - Lafuente, F. J.

AU - Solà, C.

PY - 1991/9/1

Y1 - 1991/9/1

N2 - A novel method to analyze β-galactosidase by Flow Injection Analysis is presented with a linear working range extended to at least 2150 U/mL, being the detection limit 25 U/mL with 55 samples per hour frecuency and a BSD of 0.954% versus 2.4% obtained by manual assay. The method was tested with optimal results with samples from Escherichia coli cultures producing β-galactosidase. © 1991 Chapman & Hall.

AB - A novel method to analyze β-galactosidase by Flow Injection Analysis is presented with a linear working range extended to at least 2150 U/mL, being the detection limit 25 U/mL with 55 samples per hour frecuency and a BSD of 0.954% versus 2.4% obtained by manual assay. The method was tested with optimal results with samples from Escherichia coli cultures producing β-galactosidase. © 1991 Chapman & Hall.

U2 - 10.1007/BF00185020

DO - 10.1007/BF00185020

M3 - Article

VL - 5

SP - 389

EP - 392

IS - 5

ER -

Assay of β-galactosidase activity by Flow Injection Analysis (FIA)

Abstract

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