TY - JOUR
T1 - ASPN and GJB2 are implicated in the mechanisms of invasion of ductal breast carcinomas
AU - Castellana, Bàrbara
AU - Escuin, Daniel
AU - Peiró, Gloria
AU - Garcia-Valdecasas, Bárbara
AU - Vázquez, Tania
AU - Pons, Cristina
AU - Pérez-Olabarria, Maitane
AU - Barnadas, Agustí
AU - Lerma, Enrique
PY - 2012/4/23
Y1 - 2012/4/23
N2 - The mechanism of progression from ductal carcinoma in situ (DCIS) to invasive ductal car-cinoma (IDC) remains largely unknown. We compared gene expression in tumors with simultaneous DCIS and IDC to decipher how diverse proteins participate in the local invasive process. Twenty frozen tumor specimens with concurrent, but separated, DCIS and IDC were mi-crodissected and evaluated. Total RNA was extracted and microarray analysis was performed using Affymetrix GeneChip® Human Gene 1.0 ST Arrays. Microarray data were validated by quantitative real time reverse transcription-PCR (qRT-PCR) and immunohistochemistry. Controls included seven pure in situ carcinomas, eight fragments from normal breast tissue, and a series of mouse breast carcinomas (MMTV-PyMT). Fifty-six genes were differentially expressed between DCIS and IDC samples. The genes upregulated in IDC samples, and probably associated with invasion, were related to the ep-ithelial-mesenchymal transition (ASPN, THBS2, FN1, SPARC, and COL11A1), cellular adhesion (GJB2), cell motility and progression (PLAUR, PLAU, BGN, ADAMTS16, and ENPP2), extracellular matrix degradation (MMP11, MMP13, and MMP14), and growth/proliferation (ST6GAL2). qRT-PCR confirmed the expression patterns of ASPN, GJB2, ENPP2, ST6GAL2, and TMBS10. Expression of the ASPN and GJB2 gene products was detected by immunohistochemistry in invasive carcinoma foci. The association of GJB2 protein expression with invasion was con-firmed by qRT-PCR in mouse tumors (P < 0.05). Conclusions: The upregulation of ASPN and GJB2 may play important roles in local invasion of breast ductal carcinomas. © Ivyspring International Publisher.
AB - The mechanism of progression from ductal carcinoma in situ (DCIS) to invasive ductal car-cinoma (IDC) remains largely unknown. We compared gene expression in tumors with simultaneous DCIS and IDC to decipher how diverse proteins participate in the local invasive process. Twenty frozen tumor specimens with concurrent, but separated, DCIS and IDC were mi-crodissected and evaluated. Total RNA was extracted and microarray analysis was performed using Affymetrix GeneChip® Human Gene 1.0 ST Arrays. Microarray data were validated by quantitative real time reverse transcription-PCR (qRT-PCR) and immunohistochemistry. Controls included seven pure in situ carcinomas, eight fragments from normal breast tissue, and a series of mouse breast carcinomas (MMTV-PyMT). Fifty-six genes were differentially expressed between DCIS and IDC samples. The genes upregulated in IDC samples, and probably associated with invasion, were related to the ep-ithelial-mesenchymal transition (ASPN, THBS2, FN1, SPARC, and COL11A1), cellular adhesion (GJB2), cell motility and progression (PLAUR, PLAU, BGN, ADAMTS16, and ENPP2), extracellular matrix degradation (MMP11, MMP13, and MMP14), and growth/proliferation (ST6GAL2). qRT-PCR confirmed the expression patterns of ASPN, GJB2, ENPP2, ST6GAL2, and TMBS10. Expression of the ASPN and GJB2 gene products was detected by immunohistochemistry in invasive carcinoma foci. The association of GJB2 protein expression with invasion was con-firmed by qRT-PCR in mouse tumors (P < 0.05). Conclusions: The upregulation of ASPN and GJB2 may play important roles in local invasion of breast ductal carcinomas. © Ivyspring International Publisher.
KW - ASPN
KW - Breast cancer
KW - Epitheli-al-mesenchymal transition
KW - GJB2
KW - In situ ductal carcinoma
KW - Invasive ductal carcinoma
UR - https://ddd.uab.cat/record/204679
U2 - https://doi.org/10.7150/jca.4120
DO - https://doi.org/10.7150/jca.4120
M3 - Article
VL - 3
SP - 175
EP - 183
JO - Journal of Cancer
JF - Journal of Cancer
SN - 1837-9664
ER -