Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms

Pinar Kara, Alfredo de la Escosura-Muñiz, Marisa Maltez-da Costa, Maria Guix, Mehmet Ozsoz, Arben Merkoçi

Research output: Contribution to journalArticleResearchpeer-review

73 Citations (Scopus)

Abstract

A label-free bioelectronic detection of aptamer-thrombin interaction based on electrochemical impedance spectroscopy (EIS) technique is reported. Multiwalled carbon nanotubes (MWCNTs) were used as modifiers of screen-printed carbon electrotransducers (SPCEs), showing improved characteristics compared to the bare SPCEs. 5'amino linked aptamer sequence was immobilized onto the modified SPCEs and then the binding of thrombin to aptamer sequence was monitored by EIS transduction of the resistance to charge transfer (Rct) in the presence of 5mM [Fe(CN)6]3-/4-, obtaining a detection limit of 105pM. This study represents an alternative electrochemical biosensor for the detection of proteins with interest for future applications. © 2010 Elsevier B.V.
Original languageEnglish
Pages (from-to)1715-1718
JournalBiosensors and Bioelectronics
Volume26
Issue number4
DOIs
Publication statusPublished - 15 Dec 2010

Keywords

  • Aptamer
  • Aptasensor
  • Carbon nanotubes
  • Electrochemical impedance spectroscopy
  • Screen-printed electrodes
  • Thrombin

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