Approaches to study light effects on brassinosteroid sensitivity

Sandi Paulišić, Maria José Molina-Contreras, Irma Roig-Villanova, Jaime F. Martínez-García

    Research output: Chapter in BookChapterResearchpeer-review

    Abstract

    © Springer Science+Business Media LLC 2017. Light perception and hormone signaling in plants are likely connected at multiple points. Light conditions, perceived by photoreceptors, control plant responses by altering hormone concentration, tissue sensitivity, or a combination of both. Whereas it is relatively straightforward to assess the light effects on hormone levels, hormone sensitivity is subjected to interpretation. In Arabidopsis thaliana seedlings, hypocotyl length is strongly affected by light conditions. As hypocotyl elongation also depends on brassinosteroids (BRs), assaying this response provides a valuable and easy way to measure the responsiveness of seedlings to BRs and the impact of light. We describe a simple protocol to evaluate the responsiveness of hypocotyls to commercial BRs and/or BR inhibitors under a range of light conditions. These assays can be used to establish whether light affects BR sensitivity or whether BRs affect light sensitivity. Overall, our protocol can be easily applied for deetiolation (under polychromatic or monochromatic light) and simulated shade treatments combined with BR treatments.
    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    Pages39-47
    Number of pages8
    Volume1564
    DOIs
    Publication statusPublished - 1 Jan 2017

    Keywords

    • Dark
    • Deetiolation
    • Hormone sensitivity
    • Hypocotyl length
    • Monochromatic light
    • Photoreceptors
    • Shade

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