Applications of mass spectrometry to the study of protein aggregation

Sílvia Bronsoms, Sebastián A. Trejo

Research output: Contribution to journalReview articleResearchpeer-review

2 Citations (Scopus)

Abstract

© Springer Science+Business Media New York 2015. Mass spectrometry is an analytical technique that measures the mass-to-charge ratio of charged particles. Nowadays mass spectrometry-based approaches play a pivotal role in both detection and characterization of proteins. Here we describe two applications to study insoluble proteins: (a) hydrogen/deuterium exchange combined with mass spectrometry to analyze structural properties of amyloid fi brils and (b) the screening for inhibitors of the aggregation process by matrix-assisted laser desorption/ionization time-offlight mass spectrometry.
Original languageEnglish
Pages (from-to)331-345
JournalMethods in molecular biology (Clifton, N.J.)
Volume1258
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Aggregation
  • Aβ42 amyloid peptide
  • Hydrogen/deuterium exchange
  • Inhibitor screening
  • Liquid chromatography
  • MALDI-TOF
  • Mass spectrometry

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