© Springer Science+Business Media New York 2015. Mass spectrometry is an analytical technique that measures the mass-to-charge ratio of charged particles. Nowadays mass spectrometry-based approaches play a pivotal role in both detection and characterization of proteins. Here we describe two applications to study insoluble proteins: (a) hydrogen/deuterium exchange combined with mass spectrometry to analyze structural properties of amyloid fi brils and (b) the screening for inhibitors of the aggregation process by matrix-assisted laser desorption/ionization time-offlight mass spectrometry.
|Journal||Methods in molecular biology (Clifton, N.J.)|
|Publication status||Published - 1 Jan 2015|
- Aβ42 amyloid peptide
- Hydrogen/deuterium exchange
- Inhibitor screening
- Liquid chromatography
- Mass spectrometry