Application of factorial design to the optimization of medium composition in batch cultures of Streptomyces lividans TK21 producing a hybrid antibiotic

Sarra Montserrat, Redin Iñaki, Ochin François, Godia Francesc, Casas Carles

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39 Citations (Scopus)

Abstract

The composition of the liquid medium employed to obtain a hybrid antibiotic in batch cultures of a recombinant strain of Streptomyces lividans TK21 has been studied. Starch and glutamic acid are the most appropriate carbon and nitrogen sources to support respectively cell growth and antibiotic production. A central composite experimental design has been employed to derive a statistical model of the effect of phosphate and glutamic acid on growth and antibiotic production, and an initial concentration of 10 mM phosphate and 52.8 mM glutamic acid have been found optimal to maximize the final antibiotic concentration in batch cultures. © 1993 Chapman & Hall.
Original languageEnglish
Pages (from-to)559-564
JournalBiotechnology Letters
Volume15
Issue number6
DOIs
Publication statusPublished - 1 Jun 1993

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