Application of an easy and reliable method for sulfolipid-I detection in the study of its distribution in Mycobacterium tuberculosis strains

Carlos Y. Soto, Mercè Cama, Isidre Gibert, Marina Luquin

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15 Citations (Scopus)

Abstract

Sulfolipid-I (SL-I) is a specific Mycobacterium tuberculosis glycolipid that has been involved in the mechanisms of tuberculoid infection. Until now, a limited number of M. tuberculosis strains have been studied to ascertain their SL-I content, mainly due to the laborious techniques of purification used: DEAE-cellulose column chromatography (DEAE) or extensive solvent extractions. We designed a two-dimensional thin layer chromatographic (2D-TLC) system which allows the easy and reliable detection of SL-I in small amounts of M. tuberculosis-free glycolipid extracts without previous purification. A characteristic SL-I signal was clearly identified by a differential cresyl violet metachromatic stain. Seven clinical isolates, M. tuberculosis H37Ra, H37Rv and Canetti strains were tested by DEAE and the 2D-TLC system. Identical results were found using both methodologies. The 2D-TLC methodology devised could be applied to a large number of strains to ascertain easily the distribution of SL-I in the strains of M. tuberculosis species. Copyright (C) 2000.
Original languageEnglish
Pages (from-to)103-107
JournalFEMS Microbiology Letters
Volume187
DOIs
Publication statusPublished - 1 Jun 2000

Keywords

  • Cresyl violet
  • Metachromatic stain
  • Mycobacterium tuberculosis
  • Sulfolipid-I

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