@inbook{4d4dd035470c4e9ab04d57aba4374c4c,
title = "Application of an aptamer-based proteomics assay (SOMAscan{\texttrademark}) in rat cerebrospinal fluid",
abstract = "{\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019. The exploration of the cerebrospinal fluid (CSF) through proteomics techniques might help in the search of molecular biomarkers relevant to neurological pathologies. Aiming this, we describe here a commercially available multiplexed proteomics technology based on the use of modified aptamers (SOMAscan{\texttrademark} assay). From our experience in exploring the rat CSF proteome, we detail the basic principles of this oligonucleotide-based proteomics approach, as well as the main data-processing steps to obtain relative quantitative values for proteins that could discriminate among different brain conditions, as an attempt in the search of neurological biomarkers. Finally, we give some tips on performing the SOMAscan assay and key recommendations on the verification analyses of the resulting candidate biomarkers.",
keywords = "Aptamer, CSF, Multiplex, Proteome, Rat, SOMAscan{\texttrademark}",
author = "Alba Simats and Laura Ramiro and Joan Montaner and Teresa Garc{\'i}a-Berrocoso",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9706-0_13",
language = "English",
isbn = "1064-3745",
volume = "2044",
series = "Methods in Molecular Biology",
pages = "221--231",
booktitle = "Methods in Molecular Biology",
}