Application of an aptamer-based proteomics assay (SOMAscan™) in rat cerebrospinal fluid

Alba Simats, Laura Ramiro, Joan Montaner, Teresa García-Berrocoso

Research output: Chapter in BookChapterResearchpeer-review

Abstract

© Springer Science+Business Media, LLC, part of Springer Nature 2019. The exploration of the cerebrospinal fluid (CSF) through proteomics techniques might help in the search of molecular biomarkers relevant to neurological pathologies. Aiming this, we describe here a commercially available multiplexed proteomics technology based on the use of modified aptamers (SOMAscan™ assay). From our experience in exploring the rat CSF proteome, we detail the basic principles of this oligonucleotide-based proteomics approach, as well as the main data-processing steps to obtain relative quantitative values for proteins that could discriminate among different brain conditions, as an attempt in the search of neurological biomarkers. Finally, we give some tips on performing the SOMAscan assay and key recommendations on the verification analyses of the resulting candidate biomarkers.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages221-231
Number of pages11
Volume2044
ISBN (Electronic)1940-6029
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume2044

Keywords

  • Aptamer
  • CSF
  • Multiplex
  • Proteome
  • Rat
  • SOMAscan™

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