Application of a validated high-performance liquid chromatography-mass spectrometry assay to the analysis of m- and p-hydroxybenzoylecgonine in meconium

S. Pichini, E. Marchei, R. Pacifici, M. Pellegrini, Jaime Lozano, O. García-Algar

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18 Citations (Scopus)

Abstract

A high-performance liquid chromatography (HPLC)-mass spectrometry (MS) assay, already validated for opiates and cocaine in meconium, has been re-applied for determination of m- and p-hydroxybenzoylecgonine, using nalorphine as the internal standard. Methodology included an initial extraction from the matrix by methanol and then a solid-phase extraction (SPE). A reversed-phase chromatography was used with a gradient of 1% acetic acid-acetonitrile coupled to atmospheric pressure ionization electrospray-mass spectrometry single ion monitoring mode. This method, validated in the range 0.005-1.00 μg analytes/g meconium, proved useful to identify and quantify these two metabolites in meconium samples, already tested for the presence of cocaine, benzoylecgonine and cocaethylene. A positivity of range of concentrations varied between 0.007 and 0.338 μg/g, confirming the importance of these two hydroxylated derivatives to monitor fetal exposure to cocaine. © 2005 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)151-156
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume820
Issue number1
DOIs
Publication statusPublished - 5 Jun 2005

Keywords

  • LC-MS
  • m-Hydroxybenzoylecgonine
  • Meconium
  • p-Hydroxybenzoylecgonine

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