Apolipoprotein A-II affects HDL apolipoprotein composition and HDL-mediated lipoprotein lipase activation

Josep Julve, Cristina Muñoz García, David Santos, Francisco Blanco-Vaca, Joan Carles Escolà-Gil

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Abstract

Introduction: Apolipoprotein (apo)A-II is the second most abundant HDL protein but its function remains largely unknown. Studies in humans and genetically-modified mice have demonstrated a role for apoA-II in triglyceride metabolism. The main objective of this study was to evaluate the relationship between apoA-II and HDL apolipoprotein composition, as well as HDL-mediated lipoprotein lipase (LPL) coactivation and plasma triglyceride concentration. Methods: Eleven-hour fasting blood samples were taken from 32 healthy volunteers. Anthropometric data and lipid and apolipoprotein parameters were analyzed. HDL isolated by ultracentrifugation was incubated in the presence of a triolein-based emulsion and bovine LPL. In 14 of these volunteers, an additional blood sample was taken 3. h after breakfast. Results: ApoA-II concentration was directly correlated with plasma triglycerides (R=0.55, p<0.05) and inversely correlated with the HDL-apoC-II+apoE/apoC-III ratio (R=-0.43, p<0.05). ApoA-II was also inversely correlated with HDL-mediated LPL coactivation (R=-0.35, p<0.05). ApoA-II concentration was directly correlated with plasma triglycerides 3. h after the fat-loading test (R=0.58, p<0.05). Conclusion: These results show that HDL-apoA-II levels play a crucial role in triglyceride catabolism and suggest that, at least in part, this is due to modulation of LPL activity. © 2010 Elsevier España, S.L. y SEA.
Original languageEnglish
Pages (from-to)192-197
JournalClinica e Investigacion en Arteriosclerosis
Volume22
Issue number5
DOIs
Publication statusPublished - 1 Sep 2010

Keywords

  • ApoA-II
  • ApoC
  • HDL
  • Lipoprotein lipase
  • Triglyceride

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    Julve, J., Muñoz García, C., Santos, D., Blanco-Vaca, F., & Escolà-Gil, J. C. (2010). Apolipoprotein A-II affects HDL apolipoprotein composition and HDL-mediated lipoprotein lipase activation. Clinica e Investigacion en Arteriosclerosis, 22(5), 192-197. https://doi.org/10.1016/j.arteri.2010.06.003