Background: There Are No Established Methods for the Preparation and Standardization of Fusarium Solani Antigens. This Lack of Standardization Makes It Difficult to Use These Antigens in Allergenic Diagnostic Tests. Objective: to Obtain An Appropriate Standardized Method for the Preparation of the Different Antigen Types of F. Solani. Methods: Production of Fungal Extracts, Followed by Biochemical and Immunological Characterization. Results: the Somatic Antigens Presented the Greatest Protein Content Most of These Proteins Are Common to the Metabolic and Hydrosoluble Antigens, Particularly Those Proteins at 35-39 Kda, 29-32 Kda and 15-16 Kda, As Detected by Electrophoresis and Immunoblotting. The Hydrosoluble Antigens Presented the Highest Protein Diversity; These Proteins Were the Most Specific, Showing Minor Determinants in Common with the Other Antigens. Conclusions: It Is Recommended That a Mixture of the Different Antigen Sources Be Used in Order to Obtain Extracts Which Would Cover the Maximum Number of Diagnostic Possibilities.
|Journal||Allergologia et Immunopathologia|
|Publication status||Published - 1 Sep 2000|
- Antigens characterization
- Cross reactivity
- Fusarium solani