Antigen retrieval by wet autoclaving for p53 immunostaining

Cristina Pons, Irmgard Costa, Bar von Schilling, Xavier Matias-Guiu, Jaime Prat

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

A comparative study of antigen unmasking by wet autoclaving and microwave heating so as to immunostain p53 was applied to a series of 63 ovarian and endometrial carcinomas. Wet autoclaving proved to be a rather useful method for restoring p53 antigens. One of its main advantages is the standardization of time and temperature conditions. © 1995 Lippincott–Raven Publishers. Philadelphia.
Original languageEnglish
Pages (from-to)265-267
JournalApplied Immunohistochemistry
Volume3
Issue number4
Publication statusPublished - 1 Jan 1995

Keywords

  • Antigen retrieval
  • Immunohistochemistry
  • Microwave heating
  • P53
  • Wet autoclaving

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