Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers

Juan F. Delgado; Antoni Berenguer-Llergo; Germà Julià; Gema Navarro; Mateu Espasa; Sara Rodríguez; Noemí Sánchez; Eva Van Den Eynde; Marta Navarro; Joan Calvet; Jordi Gratacós; Rosa M. Serrano; Pilar Peña; María J. Amengual

doi:https://doi.org/10.3390/v14061235

Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers

Juan F. Delgado^*, Antoni Berenguer-Llergo, Germà Julià, Gema Navarro, Mateu Espasa, Sara Rodríguez, Noemí Sánchez, Eva Van Den Eynde, Marta Navarro, Joan Calvet, Jordi Gratacós, Rosa M. Serrano, Pilar Peña, María J. Amengual

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

The aim of this study was to characterize the antibody response induced by SARS-CoV-2 mRNA vaccines in a cohort of healthcare workers. A total of 2247 serum samples were analyzed using the Elecsys® Anti-SARS-CoV-2 S-test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland). Sex, age, body mass index (BMI), arterial hypertension, smoking and time between infection and/or vaccination and serology were considered the confounding factors. Regarding the medians, subjects previously infected with SARS-CoV-2 who preserved their response to the nucleocapsid (N) protein showed higher humoral immunogenicity (BNT162b2: 6456.0 U/mL median; mRNA-1273: 2505.0 U/mL) compared with non-infected (BNT162b2: 867.0 U/mL; mRNA-1273: 2300.5 U/mL) and infected subjects with a lost response to N protein (BNT162b2: 2992.0 U/mL). After controlling for the confounders, a higher response was still observed for mRNA-1273 compared with BNT162b2 in uninfected individuals (FC = 2.35, p < 0.0001) but not in previously infected subjects (1.11 FC, p = 0.1862). The lowest levels of antibodies were detected in previously infected non-vaccinated individuals (39.4 U/mL). Clinical variables previously linked to poor prognoses regarding SARS-CoV-2 infection, such as age, BMI and arterial hypertension, were positively associated with increasing levels of anti-S protein antibody exclusively in infected subjects. The mRNA-1273 vaccine generated a higher antibody response to the S protein than BNT162b2 in non-infected subjects only.

Original language	English
Article number	1235
Journal	Viruses
Volume	14
Issue number	6
DOIs	https://doi.org/10.3390/v14061235
Publication status	Published - Jun 2022

Keywords

BNT162b2
SARS-CoV-2
antibodies
mRNA-1273
vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

https://doi.org/10.3390/v14061235

https://ddd.uab.cat/record/259907

Cite this

Delgado, J. F., Berenguer-Llergo, A., Julià, G., Navarro, G., Espasa, M., Rodríguez, S., Sánchez, N., Van Den Eynde, E., Navarro, M., Calvet, J., Gratacós, J., Serrano, R. M., Peña, P., & Amengual, M. J. (2022). Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers. Viruses, 14(6), Article 1235. https://doi.org/10.3390/v14061235

@article{fae1fe0f41384e6398bbc308fa736526,

title = "Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers",

abstract = "The aim of this study was to characterize the antibody response induced by SARS-CoV-2 mRNA vaccines in a cohort of healthcare workers. A total of 2247 serum samples were analyzed using the Elecsys{\textregistered} Anti-SARS-CoV-2 S-test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland). Sex, age, body mass index (BMI), arterial hypertension, smoking and time between infection and/or vaccination and serology were considered the confounding factors. Regarding the medians, subjects previously infected with SARS-CoV-2 who preserved their response to the nucleocapsid (N) protein showed higher humoral immunogenicity (BNT162b2: 6456.0 U/mL median; mRNA-1273: 2505.0 U/mL) compared with non-infected (BNT162b2: 867.0 U/mL; mRNA-1273: 2300.5 U/mL) and infected subjects with a lost response to N protein (BNT162b2: 2992.0 U/mL). After controlling for the confounders, a higher response was still observed for mRNA-1273 compared with BNT162b2 in uninfected individuals (FC = 2.35, p < 0.0001) but not in previously infected subjects (1.11 FC, p = 0.1862). The lowest levels of antibodies were detected in previously infected non-vaccinated individuals (39.4 U/mL). Clinical variables previously linked to poor prognoses regarding SARS-CoV-2 infection, such as age, BMI and arterial hypertension, were positively associated with increasing levels of anti-S protein antibody exclusively in infected subjects. The mRNA-1273 vaccine generated a higher antibody response to the S protein than BNT162b2 in non-infected subjects only.",

keywords = "BNT162b2, SARS-CoV-2, antibodies, mRNA-1273, vaccine",

author = "Delgado, {Juan F.} and Antoni Berenguer-Llergo and Germ{\`a} Juli{\`a} and Gema Navarro and Mateu Espasa and Sara Rodr{\'i}guez and Noem{\'i} S{\'a}nchez and {Van Den Eynde}, Eva and Marta Navarro and Joan Calvet and Jordi Gratac{\'o}s and Serrano, {Rosa M.} and Pilar Pe{\~n}a and Amengual, {Mar{\'i}a J.}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = jun,

doi = "https://doi.org/10.3390/v14061235",

language = "English",

volume = "14",

journal = "Viruses",

issn = "1999-4915",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "6",

}

Delgado, JF, Berenguer-Llergo, A, Julià, G, Navarro, G, Espasa, M, Rodríguez, S, Sánchez, N, Van Den Eynde, E, Navarro, M, Calvet, J, Gratacós, J, Serrano, RM, Peña, P & Amengual, MJ 2022, 'Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers', Viruses, vol. 14, no. 6, 1235. https://doi.org/10.3390/v14061235

TY - JOUR

T1 - Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers

AU - Delgado, Juan F.

AU - Berenguer-Llergo, Antoni

AU - Julià, Germà

AU - Navarro, Gema

AU - Espasa, Mateu

AU - Rodríguez, Sara

AU - Sánchez, Noemí

AU - Van Den Eynde, Eva

AU - Navarro, Marta

AU - Calvet, Joan

AU - Gratacós, Jordi

AU - Serrano, Rosa M.

AU - Peña, Pilar

AU - Amengual, María J.

PY - 2022/6

Y1 - 2022/6

N2 - The aim of this study was to characterize the antibody response induced by SARS-CoV-2 mRNA vaccines in a cohort of healthcare workers. A total of 2247 serum samples were analyzed using the Elecsys® Anti-SARS-CoV-2 S-test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland). Sex, age, body mass index (BMI), arterial hypertension, smoking and time between infection and/or vaccination and serology were considered the confounding factors. Regarding the medians, subjects previously infected with SARS-CoV-2 who preserved their response to the nucleocapsid (N) protein showed higher humoral immunogenicity (BNT162b2: 6456.0 U/mL median; mRNA-1273: 2505.0 U/mL) compared with non-infected (BNT162b2: 867.0 U/mL; mRNA-1273: 2300.5 U/mL) and infected subjects with a lost response to N protein (BNT162b2: 2992.0 U/mL). After controlling for the confounders, a higher response was still observed for mRNA-1273 compared with BNT162b2 in uninfected individuals (FC = 2.35, p < 0.0001) but not in previously infected subjects (1.11 FC, p = 0.1862). The lowest levels of antibodies were detected in previously infected non-vaccinated individuals (39.4 U/mL). Clinical variables previously linked to poor prognoses regarding SARS-CoV-2 infection, such as age, BMI and arterial hypertension, were positively associated with increasing levels of anti-S protein antibody exclusively in infected subjects. The mRNA-1273 vaccine generated a higher antibody response to the S protein than BNT162b2 in non-infected subjects only.

AB - The aim of this study was to characterize the antibody response induced by SARS-CoV-2 mRNA vaccines in a cohort of healthcare workers. A total of 2247 serum samples were analyzed using the Elecsys® Anti-SARS-CoV-2 S-test (Roche Diagnostics International Ltd., Rotkreuz, Switzerland). Sex, age, body mass index (BMI), arterial hypertension, smoking and time between infection and/or vaccination and serology were considered the confounding factors. Regarding the medians, subjects previously infected with SARS-CoV-2 who preserved their response to the nucleocapsid (N) protein showed higher humoral immunogenicity (BNT162b2: 6456.0 U/mL median; mRNA-1273: 2505.0 U/mL) compared with non-infected (BNT162b2: 867.0 U/mL; mRNA-1273: 2300.5 U/mL) and infected subjects with a lost response to N protein (BNT162b2: 2992.0 U/mL). After controlling for the confounders, a higher response was still observed for mRNA-1273 compared with BNT162b2 in uninfected individuals (FC = 2.35, p < 0.0001) but not in previously infected subjects (1.11 FC, p = 0.1862). The lowest levels of antibodies were detected in previously infected non-vaccinated individuals (39.4 U/mL). Clinical variables previously linked to poor prognoses regarding SARS-CoV-2 infection, such as age, BMI and arterial hypertension, were positively associated with increasing levels of anti-S protein antibody exclusively in infected subjects. The mRNA-1273 vaccine generated a higher antibody response to the S protein than BNT162b2 in non-infected subjects only.

KW - BNT162b2

KW - SARS-CoV-2

KW - antibodies

KW - mRNA-1273

KW - vaccine

UR - http://www.scopus.com/inward/record.url?scp=85132201386&partnerID=8YFLogxK

U2 - https://doi.org/10.3390/v14061235

DO - https://doi.org/10.3390/v14061235

M3 - Article

C2 - 35746706

AN - SCOPUS:85132201386

SN - 1999-4915

VL - 14

JO - Viruses

JF - Viruses

IS - 6

M1 - 1235

ER -

Antibody Response Induced by BNT162b2 and mRNA-1273 Vaccines against the SARS-CoV-2 in a Cohort of Healthcare Workers

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this