Anti-HEV antibodies in domestic animal species and rodents from Spain using a genotype 3-based ELISA

Bibiana Peralta, Maribel Casas, Nilsa de Deus, Marga Martín, Anna Ortuño, Eva Pérez-Martín, Sonia Pina, Enric Mateu

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44 Citations (Scopus)

Abstract

A truncated ORF2 capsid HEV antigen derived from a genotype 3 strain was developed in insect cells and insect larvae, and compared with the Sar55 antigen and a commercial ELISA. The antigen expressed in insect cells showed a better correlation with Sar55 (kappa value (k) = 0.84) than the insect larvae antigen (k = 0.69), and a better reproducibility as indicated by the intra and interplate variation coefficients. Commercial ELISA designed for human diagnosis but adapted to animal use using specific secondary antibodies demonstrated to have a very low sensitivity. The insect cell expressed antigen was used to develop an ELISA to detect anti-HEV-IgG in serum samples of different domestic animal and rodents. Seropositivity in the studied animal populations was 71.4% for pigs, 0.60% for goats, 1.92% for sheep, and 11.11% for cats. None of the 1170 cattle samples or 166 rodent samples analyzed was positive. © 2009 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)66-73
JournalVeterinary Microbiology
Volume137
DOIs
Publication statusPublished - 28 May 2009

Keywords

  • Domestic animals
  • ELISA
  • Genotype 3
  • HEV
  • Rodents

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