Analytical performance and clinical usefulness of two binding assays for growth hormone binding protein (GHBP) measurement: High performance liquid chromatography (HPLC)-gel filtration and dextran-coated charcoal adsorption

Maria Antonia Llopis, Maria Luisa Granada, Laura Audí, Anna Sanmartí, Joan Bel, Luis Sánchez-Planell, Xavier Formiguera, Fernando Marin, August Corominas

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9 Citations (Scopus)

Abstract

We compared two binding assays for growth hormone binding protein (GHBP) measurements, which differ in the method of bound and free GH separation: HPLC-gel filtration or dextran coated-charcoal adsorption (DCC). Two pools of sera (high and medium GHBP activity) were used for quality-control assessment. Moreover, 62 samples from 34 children and 28 adults with different nutritional status were studied. Total, between- and intra-iodination coefficients of variation (CVs) from the two methods were not different. Although percentage binding measured in the pool sera significantly differed, the concentrations assessed by Scatchard plot were comparable. Results obtained by the two methods in the 62 sera were significantly correlated (r = 0.77, P < 0.001). With both methods GHBP activity correlated with chronological age and body mass index (BMI) and differed among groups with different nutritional status. Although HPLC and DCC separation methods for GHBP measurement differ in their practicability, our study demonstrates that performance and the clinical usefulness of the two methods are comparable.
Original languageEnglish
Pages (from-to)167-181
JournalClinica Chimica Acta
Volume267
Issue number2
DOIs
Publication statusPublished - 28 Nov 1997

Keywords

  • Dextran-coated charcoal adsorption
  • Growth hormone binding protein (GHBP)
  • High performance liquid chromatography (HPLC)
  • Idiopathic short Stature
  • Obesity

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