TY - JOUR
T1 - Analytical approaches for assessing aggregation of protein biopharmaceuticals
AU - Villaverde Corrales, Antonio Pedro
AU - Ferrer Miralles, Neus
AU - Vazquez Gomez, Esther
AU - García-Fruitós, Elena
AU - Vazquez, Esther
AU - Gonzalez-Montalbán, Nuria
AU - Ferrer-Miralles, Neus
AU - Villaverde, Antonio
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Production of protein-based pharmaceuticals is a major issue in conventional pharmacology, biomedicine and nanomedicine. Being mostly obtained by genetic engineering, the quality and activity of protein drugs is a steady matter of concern. Although the physiology of the host recombinant cells, mostly mammalian and microbial, is progressively understood, the complexity of the cellular quality control systems escapes rational protein and process engineering, and recombinant proteins are often unstable, aggregate and/or do not reach the fully native conformation compatible with proper biological activity. In this review, we summarize the main biological aspects of protein folding and misfolding, mainly focusing in microbial cells, the newest insights in the biological control of protein quality and the main and analytical approaches that are suitable for the fast evaluation of the conformational quality and aggregation of recombinant drugs, even if showing apparent solubility. © 2011 Bentham Science Publishers.
AB - Production of protein-based pharmaceuticals is a major issue in conventional pharmacology, biomedicine and nanomedicine. Being mostly obtained by genetic engineering, the quality and activity of protein drugs is a steady matter of concern. Although the physiology of the host recombinant cells, mostly mammalian and microbial, is progressively understood, the complexity of the cellular quality control systems escapes rational protein and process engineering, and recombinant proteins are often unstable, aggregate and/or do not reach the fully native conformation compatible with proper biological activity. In this review, we summarize the main biological aspects of protein folding and misfolding, mainly focusing in microbial cells, the newest insights in the biological control of protein quality and the main and analytical approaches that are suitable for the fast evaluation of the conformational quality and aggregation of recombinant drugs, even if showing apparent solubility. © 2011 Bentham Science Publishers.
KW - Aggregation
KW - Analytical procedures
KW - Protein drugs
KW - Recombinant proteins
KW - Screening
U2 - 10.2174/138920111798357339
DO - 10.2174/138920111798357339
M3 - Article
VL - 12
SP - 1530
EP - 1536
JO - Current Pharmaceutical Biotechnology
JF - Current Pharmaceutical Biotechnology
SN - 1389-2010
IS - 10
ER -