Analysis of the Spanish CCR5-Δ32 inventory of cord blood units: Lower cell counts in homozygous donors

Emma Enrich, Francisco Vidal, Francisco Sánchez-Gordo, Juan M. Gómez-Zumaquero, Antonio Balas, Francesc Rudilla, Luisa Barea, Ana Castro, Luis Larrea, Miguel A. Perez-Vaquero, Isidro Prat, Sergio Querol, Gregorio Garrido, Rafael Matesanz, Enric Carreras, Rafael F. Duarte

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2 Citations (Scopus)

Abstract

© 2018 Macmillan Publishers Limited, part of Springer Nature. The possibility to use CCR5-Δ32 umbilical cord blood to cure HIV infection in patients in need of a hematopoietic transplant has been suggested. The less stringent HLA compatibility needed in this type of transplant facilitates the search of a suitable donor having the CCR5-Δ32 mutation. To achieve an inventory of CCR5-Δ32 cord blood units, the 20,236 best cell quality units of the Spanish Registry were genotyped. Furthermore, their CD34 + and total nucleated cells counts, blood type, gender, HLA and donor's geographical and ancestral origin were analyzed. The results showed 130 (0.64%) units homozygous for the deletion, 2,646 (13.08%) heterozygous and 17,460 (86.28%) did not present the mutation. Interestingly, a significant lower amount of CD34 + cells was found in the CCR5-Δ32 homozygous units. In addition, a significant association was found among donor's ancestral origin and the mutation, with a higher percentage of CCR5-Δ32 units with a European ancestry. In summary, identification of a relatively high number of CCR5-Δ32 units is feasible and will facilitate the development of clinical trials for HIV cure in patients requiring hematopoietic transplantation. Further studies are required to understand the significance of lower cell counts within the CCR5-Δ32 homozygous group and its clinical impact.
Original languageEnglish
Pages (from-to)741-748
JournalBone Marrow Transplantation
Volume53
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018

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    Enrich, E., Vidal, F., Sánchez-Gordo, F., Gómez-Zumaquero, J. M., Balas, A., Rudilla, F., Barea, L., Castro, A., Larrea, L., Perez-Vaquero, M. A., Prat, I., Querol, S., Garrido, G., Matesanz, R., Carreras, E., & Duarte, R. F. (2018). Analysis of the Spanish CCR5-Δ32 inventory of cord blood units: Lower cell counts in homozygous donors. Bone Marrow Transplantation, 53(6), 741-748. https://doi.org/10.1038/s41409-018-0114-8