TY - JOUR
T1 - Analysis of the HLA class 1 associated peptide repertoire in a hepatocellular carcinoma cell line reveals tumor-specific peptides as putative targets for immunotherapy
AU - Alvarez Perez, Ignacio Gerardo
AU - Carrascal, Montserrat
AU - Canals, Francesc
AU - Muixí, Laia
AU - Abián, Joaquín
AU - Jaraquemada, Dolores
PY - 2007/3/1
Y1 - 2007/3/1
N2 - HLA class I molecules present peptides on the cell surface to CD8+ T cells. The repertoire of peptides that associate to class 1 molecules represents the cellular proteome. Therefore, cells expressing different proteomes could generate different class I-associated peptide repertoires. A large number of peptides have been sequenced from HLA class I alleles, mostly from lymphoid cells. On the other hand, T cell immunotherapy is a goal in the fight against cancer, but the identification of T cell epitopes is a laborious task. Proteomic techniques allow the definition of putative T cell epitopes by the identification of HLA natural ligands in tumor cells. In this study, we have compared the HLA class I-associated peptide repertoire from the hepatocellular carcinoma (HCC) cell line SK-Hep-1 with that previously described from lymphoid cells. The analysis ofthe peptide pool confirmed that, as expected, the peptides from SK-Hep-1 derive from proteins localized in the same compartments as in lymphoid cells. Within this pool, we have identified 12 HLA class I peptides derived from HCC-related proteins. This confirms that tumor cell lines could be a good source of tumor associated antigens to be used, together with MS, to define putative epitopes for cytotoxic T cells from cancer patients. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
AB - HLA class I molecules present peptides on the cell surface to CD8+ T cells. The repertoire of peptides that associate to class 1 molecules represents the cellular proteome. Therefore, cells expressing different proteomes could generate different class I-associated peptide repertoires. A large number of peptides have been sequenced from HLA class I alleles, mostly from lymphoid cells. On the other hand, T cell immunotherapy is a goal in the fight against cancer, but the identification of T cell epitopes is a laborious task. Proteomic techniques allow the definition of putative T cell epitopes by the identification of HLA natural ligands in tumor cells. In this study, we have compared the HLA class I-associated peptide repertoire from the hepatocellular carcinoma (HCC) cell line SK-Hep-1 with that previously described from lymphoid cells. The analysis ofthe peptide pool confirmed that, as expected, the peptides from SK-Hep-1 derive from proteins localized in the same compartments as in lymphoid cells. Within this pool, we have identified 12 HLA class I peptides derived from HCC-related proteins. This confirms that tumor cell lines could be a good source of tumor associated antigens to be used, together with MS, to define putative epitopes for cytotoxic T cells from cancer patients. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
KW - HLA
KW - Mass spectrometry
KW - Peptides
KW - Tumor
U2 - 10.1002/prca.200600388
DO - 10.1002/prca.200600388
M3 - Article
VL - 1
SP - 286
EP - 298
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
SN - 1862-8346
IS - 3
ER -