Analysis of the HLA class 1 associated peptide repertoire in a hepatocellular carcinoma cell line reveals tumor-specific peptides as putative targets for immunotherapy

Ignacio Gerardo Alvarez Perez, Montserrat Carrascal, Francesc Canals, Laia Muixí, Joaquín Abián, Dolores Jaraquemada

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

HLA class I molecules present peptides on the cell surface to CD8+ T cells. The repertoire of peptides that associate to class 1 molecules represents the cellular proteome. Therefore, cells expressing different proteomes could generate different class I-associated peptide repertoires. A large number of peptides have been sequenced from HLA class I alleles, mostly from lymphoid cells. On the other hand, T cell immunotherapy is a goal in the fight against cancer, but the identification of T cell epitopes is a laborious task. Proteomic techniques allow the definition of putative T cell epitopes by the identification of HLA natural ligands in tumor cells. In this study, we have compared the HLA class I-associated peptide repertoire from the hepatocellular carcinoma (HCC) cell line SK-Hep-1 with that previously described from lymphoid cells. The analysis ofthe peptide pool confirmed that, as expected, the peptides from SK-Hep-1 derive from proteins localized in the same compartments as in lymphoid cells. Within this pool, we have identified 12 HLA class I peptides derived from HCC-related proteins. This confirms that tumor cell lines could be a good source of tumor associated antigens to be used, together with MS, to define putative epitopes for cytotoxic T cells from cancer patients. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)286-298
JournalProteomics - Clinical Applications
Volume1
Issue number3
DOIs
Publication statusPublished - 1 Mar 2007

Keywords

  • HLA
  • Mass spectrometry
  • Peptides
  • Tumor

Fingerprint Dive into the research topics of 'Analysis of the HLA class 1 associated peptide repertoire in a hepatocellular carcinoma cell line reveals tumor-specific peptides as putative targets for immunotherapy'. Together they form a unique fingerprint.

Cite this