Analysis of parainflammation in chronic glaucoma using vitreous-oct imaging

María Jesús Rodrigo*, Manuel Subías, Alberto Montolío, Silvia Méndez-Martínez, Teresa Martínez-Rincón, Lorena Arias, David García-Herranz, Irene Bravo-Osuna, Julian Garcia-Feijoo, Luis Pablo, José Cegoñino, Rocio Herrero-Vanrell, Ana Carretero, Jesus Ruberte, Elena Garcia-Martin, Amaya Pérez Del Palomar

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Glaucoma causes blindness due to the progressive death of retinal ganglion cells. The immune response chronically and subclinically mediates a homeostatic role. In current clinical practice, it is impossible to analyse neuroinflammation non-invasively. However, analysis of vitreous images using optical coherence tomography detects the immune response as hyperreflective opacities. This study monitors vitreous parainflammation in two animal models of glaucoma, comparing both healthy controls and sexes over six months. Computational analysis characterizes in vivo the hyperreflective opacities, identified histologically as hyalocyte-like Iba-1+ (microglial marker) cells. Glaucomatous eyes showed greater intensity and number of vitreous opacities as well as dynamic fluctuations in the percentage of activated cells (50–250 microns2 ) vs. non-activated cells (10–50 microns2 ), isolated cells (10 microns2) and complexes (>250 microns2 ). Smaller opacities (isolated cells) showed the highest mean intensity (intracellular machinery), were the most rounded at earlier stages (recruitment) and showed the greatest change in orientation (motility). Study of vitreous parainflammation could be a biomarker of glaucoma onset and progression.

Original languageEnglish
Article number1792
JournalBiomedicines
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Animal models
  • Biomedical imaging
  • Glaucoma
  • Image analysis and processing
  • Inflammation
  • Optical coherence tomography
  • Vitreous body

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