Analysis of glutathione and vitamin C effects on the benzenetriol-induced DNA damage in isolated human lymphocytes

M. Pitarque, A. Creus, R. Marcos

Research output: Contribution to journalReview articleResearchpeer-review

3 Citations (Scopus)

Abstract

The alkaline single-cell gel electrophoresis (or comet) assay was applied to evaluate the eventual DNA damage induced by the triphenolic metabolite of benzene, 1,2,4-benzenetriol (BT), in isolated human lymphocytes. Prior to BT treatment, ranging from 5-50 μM, a supplementation with glutathione (GSH, 350 μg/ml) was carried out to assess whether GSH may have a modulating effect on the comet response. The effect of a fixed dose of BT was also evaluated in the presence of the exogenous antioxidant vitamin C (40 and 200 μM). Additionally, we investigated whether the polymorphism of glutathione S-transferase T1 (GSTT1) gene may affect the individual level of BT-induced DNA damage in vitro. For all donors included in the present study, BT produced a significant dose-response relationship. No clear effect of GSH preincubation was seen on the BT-induced response. On the contrary, a significant reduction of DNA damage was observed in the presence of vitamin C (at least at 200 μM). Although our data suggest some individual differences according to the GSTT1 genotype in the outcome of the comet assay, a large number of individuals should be studied in further investigations to obtain reliable conclusions. ©2006 with author. Published by TheScientificWorld, Ltd.
Original languageEnglish
Pages (from-to)1191-1201
JournalThe Scientific World Journal
Volume6
DOIs
Publication statusPublished - 25 Sep 2006

Keywords

  • Benzenetriol
  • Comet assay
  • Glutathione
  • Oxidative DNA damage
  • Vitamin C

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