TY - JOUR
T1 - An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels
AU - Haagmans, Bart L.
AU - Van Den Brand, Judith M.A.
AU - Raj, V. Stalin
AU - Volz, Asisa
AU - Wohlsein, Peter
AU - Smits, Saskia L.
AU - Schipper, Debby
AU - Bestebroer, Theo M.
AU - Okba, Nisreen
AU - Fux, Robert
AU - Bensaid, Albert
AU - Foz, David Solanes
AU - Kuiken, Thijs
AU - Baumgärtner, Wolfgang
AU - Segalés, Joaquim
AU - Sutter, Gerd
AU - Osterhaus, Albert D.M.E.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Middle East respiratory syndrome coronavirus (MERS-CoV) infections have led to an ongoing outbreak in humans, which was fueled by multiple zoonotic MERS-CoV introductions from dromedary camels. In addition to the implementation of hygiene measures to limit further camel-to-human and human-to-human transmissions, vaccine-mediated reduction of MERS-CoV spread from the animal reservoir may be envisaged. Here we show that a modified vaccinia virus Ankara (MVA) vaccine expressing the MERS-CoV spike protein confers mucosal immunity in dromedary camels. Compared with results for control animals, we observed a significant reduction of excreted infectious virus and viral RNA transcripts in vaccinated animals upon MERS-CoV challenge. Protection correlated with the presence of serum neutralizing antibodies to MERS-CoV. Induction of MVA-specific antibodies that cross-neutralize camelpox virus would also provide protection against camelpox.
AB - Middle East respiratory syndrome coronavirus (MERS-CoV) infections have led to an ongoing outbreak in humans, which was fueled by multiple zoonotic MERS-CoV introductions from dromedary camels. In addition to the implementation of hygiene measures to limit further camel-to-human and human-to-human transmissions, vaccine-mediated reduction of MERS-CoV spread from the animal reservoir may be envisaged. Here we show that a modified vaccinia virus Ankara (MVA) vaccine expressing the MERS-CoV spike protein confers mucosal immunity in dromedary camels. Compared with results for control animals, we observed a significant reduction of excreted infectious virus and viral RNA transcripts in vaccinated animals upon MERS-CoV challenge. Protection correlated with the presence of serum neutralizing antibodies to MERS-CoV. Induction of MVA-specific antibodies that cross-neutralize camelpox virus would also provide protection against camelpox.
U2 - 10.1126/science.aad1283
DO - 10.1126/science.aad1283
M3 - Article
SN - 0036-8075
VL - 351
SP - 77
EP - 81
JO - Science
JF - Science
ER -
