An orf from Bacillus licheniformis encodes a putative DNA repressor

Jordi Naval, Daniel Aguilar, Xavier Serra, Josep A. Pérez-Pons, Jaume Piñol, Jorge Lloberas, Enrique Querol

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The complete sequence of a reading frame adjacent to the endo-β-1,3-1,4-D-glucanase gene from Bacillus licheniformis is reported. It encodes a putative 171 amino acid residues protein with either, low significant sequence similarity in data banks or the corresponding orthologue in the recently sequenced Bacillus subtilis genome. Computer analyses predict a canonical Helix-Turn-Helix motif characteristic of bacterial repressors/DNA binding proteins. A maxicells assay shows that the encoded polypeptide is expressed. A DNA-protein binding. assay performed by gel electrophoresis shows that the expressed protein specifically binds to Bacillus licheniformis DNA. © 2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Original languageEnglish
Pages (from-to)1-7
JournalMitochondrial DNA
Volume11
DOIs
Publication statusPublished - 1 Dec 2000

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