The complete sequence of a reading frame adjacent to the endo-β-1,3-1,4-D-glucanase gene from Bacillus licheniformis is reported. It encodes a putative 171 amino acid residues protein with either, low significant sequence similarity in data banks or the corresponding orthologue in the recently sequenced Bacillus subtilis genome. Computer analyses predict a canonical Helix-Turn-Helix motif characteristic of bacterial repressors/DNA binding proteins. A maxicells assay shows that the encoded polypeptide is expressed. A DNA-protein binding. assay performed by gel electrophoresis shows that the expressed protein specifically binds to Bacillus licheniformis DNA. © 2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.