An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection

Carmen C. Mayorga-Martinez, Alejandro Chamorro-García, Lorena Serrano, Lourdes Rivas, Daniel Quesada-Gonzalez, Laura Altet, Olga Francino, Armand Sánchez, Arben Merkoçi

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

© The Royal Society of Chemistry. An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO<inf>2</inf> NP). An aminated oligonucleotide probe is immobilized on the IrO<inf>2</inf> NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
Original languageEnglish
Pages (from-to)5166-5171
JournalJournal of Materials Chemistry B
Volume3
DOIs
Publication statusPublished - 14 Jul 2015

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