An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936–1939)

Francesc Calafell, Roger Anglada, Núria Bonet, Mercedes González-Ruiz, Gemma Prats-Muñoz, Raquel Rasal, Carles Lalueza-Fox, Jaume Bertranpetit, Assumpció Malgosa, Ferran Casals

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Next-generation sequencing technologies have opened new opportunities in forensic genetics. Here, we assess the applicability and performance of the MiSeq FGx™ & ForenSeq™ DNA Signature Prep Kit (Illumina) for the identification of individuals from the mass graves of the Spanish Civil War (1936–1939). The main limitations for individual identification are the low number of possible first-degree living relatives and the high levels of DNA degradation reported in previous studies. Massively parallel sequencing technologies enabling the analysis of hundreds of regions and prioritizing short length amplicons constitute a promising tool for this kind of approaches. In this study, we first explore the power of this new technology to detect first- and second-degree kinship given different scenarios of DNA degradation. Second, we specifically assess its performance in a set of low DNA input samples previously analyzed with CE technologies. We conclude that this methodology will allow identification of up to second-degree relatives, even in situations with low sequencing performance and important levels of allele drop-out; it is thus a technology that resolves previous drawbacks and that will allow a successful approximation to the identification of remains.
Original languageEnglish
Pages (from-to)2841-2847
JournalElectrophoresis
Volume37
Issue number21
DOIs
Publication statusPublished - 1 Oct 2016

Keywords

  • Low input DNA
  • Mass graves
  • Massively parallel sequencing
  • Next-generation sequencing
  • Spanish Civil War

Fingerprint Dive into the research topics of 'An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936–1939)'. Together they form a unique fingerprint.

  • Cite this

    Calafell, F., Anglada, R., Bonet, N., González-Ruiz, M., Prats-Muñoz, G., Rasal, R., Lalueza-Fox, C., Bertranpetit, J., Malgosa, A., & Casals, F. (2016). An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936–1939). Electrophoresis, 37(21), 2841-2847. https://doi.org/10.1002/elps.201600180