TY - JOUR
T1 - Amperometric thyroxine sensor using a nanocomposite based on graphene modified with gold nanoparticles carrying a thiolated β-cyclodextrin
AU - Muñoz, Jose
AU - Riba-Moliner, Marta
AU - Brennan, Lorcan J.
AU - Gun’ko, Yurii K.
AU - Céspedes, Francisco
AU - González-Campo, Arántzazu
AU - Baeza, Mireia
PY - 2016/5/1
Y1 - 2016/5/1
N2 - © 2016, Springer-Verlag Wien. This article reports a novel electrochemical sensor based on a nanocomposite for the sensitive determination of Thyroxine (T4), the active form of the hormone. Hydrodynamic amperometry is performed with a nanocomposite electrode based on the dispersion of a graphene–based filler hybrid-nanomaterial throughout an insulating epoxy resin in the optimum composition ratio (the near–percolation composition). This hybrid-nanomaterial consists of reduced graphene oxide tuned with gold nanoparticles and a biorecognition agent, the thiolated β-cyclodextrin. Recognition of T4 is accomplished via supramolecular chemistry, due to the formation of an inclusion complex between β-cyclodextrin and T4. The amperometric device operates at +0.85 V vs. Ag/AgCl, where the oxidation of T4 takes place on the electrode surface. The sensor covers the 1.00 nM to 14 nM T4concentration range in a 0.1 M HCl solution, with a detection limit of 1.00 ± 0.02 nM. The sensor can be easily reset by polishing. It exhibits the lowest detection limit regarding to any other electrochemical electrodes for T4 determination previously described in literature. [Figure not available: see fulltext.]
AB - © 2016, Springer-Verlag Wien. This article reports a novel electrochemical sensor based on a nanocomposite for the sensitive determination of Thyroxine (T4), the active form of the hormone. Hydrodynamic amperometry is performed with a nanocomposite electrode based on the dispersion of a graphene–based filler hybrid-nanomaterial throughout an insulating epoxy resin in the optimum composition ratio (the near–percolation composition). This hybrid-nanomaterial consists of reduced graphene oxide tuned with gold nanoparticles and a biorecognition agent, the thiolated β-cyclodextrin. Recognition of T4 is accomplished via supramolecular chemistry, due to the formation of an inclusion complex between β-cyclodextrin and T4. The amperometric device operates at +0.85 V vs. Ag/AgCl, where the oxidation of T4 takes place on the electrode surface. The sensor covers the 1.00 nM to 14 nM T4concentration range in a 0.1 M HCl solution, with a detection limit of 1.00 ± 0.02 nM. The sensor can be easily reset by polishing. It exhibits the lowest detection limit regarding to any other electrochemical electrodes for T4 determination previously described in literature. [Figure not available: see fulltext.]
KW - Biorecognition
KW - Carbon filler
KW - Epoxy resin
KW - High-Resolution Transmission Electron Microscopy
KW - Nanocomposite
KW - Near-percolation composition
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000374980800006&KeyUID=WOS:000374980800006
U2 - 10.1007/s00604-016-1783-x
DO - 10.1007/s00604-016-1783-x
M3 - Article
VL - 183
SP - 1579
EP - 1589
IS - 5
ER -