Amperometric immunosensors based on rigid conducting immunocomposites

Marta Santandreu; Francisco Cespedes; Salvador Alegret; Esteve Martinez-Fabregas

doi:10.1021/ac961222b

Amperometric immunosensors based on rigid conducting immunocomposites

Marta Santandreu, Francisco Cespedes, Salvador Alegret, Esteve Martinez-Fabregas

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

103 Citations (Scopus)

Abstract

Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgCl). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay. © 1997 American Chemical Society.

Original language	English
Pages (from-to)	2080-2085
Journal	Analytical Chemistry
Volume	69
Issue number	12
DOIs	https://doi.org/10.1021/ac961222b
Publication status	Published - 1 Dec 1997

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title = "Amperometric immunosensors based on rigid conducting immunocomposites",

abstract = "Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgCl). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay. {\textcopyright} 1997 American Chemical Society.",

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AU - Cespedes, Francisco

AU - Alegret, Salvador

AU - Martinez-Fabregas, Esteve

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AB - Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgCl). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay. © 1997 American Chemical Society.

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