TY - JOUR
T1 - Amperometric immunosensors based on rigid conducting immunocomposites
AU - Santandreu, Marta
AU - Cespedes, Francisco
AU - Alegret, Salvador
AU - Martinez-Fabregas, Esteve
PY - 1997/12/1
Y1 - 1997/12/1
N2 - Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgCl). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay. © 1997 American Chemical Society.
AB - Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins. This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgCl). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay. © 1997 American Chemical Society.
U2 - 10.1021/ac961222b
DO - 10.1021/ac961222b
M3 - Article
SN - 0003-2700
VL - 69
SP - 2080
EP - 2085
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 12
ER -