Amperometric glucose biosensor based on an electrocatalytically bulk-modified epoxy-graphite biocomposite

F. Céspedes; E. Martínez-Fàbregas; S. Alegret

doi:10.1016/0003-2670(93)80003-4

Amperometric glucose biosensor based on an electrocatalytically bulk-modified epoxy-graphite biocomposite

F. Céspedes, E. Martínez-Fàbregas, S. Alegret

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

40 Citations (Scopus)

Abstract

An inexpensive, robust, polishable and easily machinable amperometric glucose transducer was constructed. The biocomposite materials used were graphite, palladium-gold, non-conducting epoxy resin and glucose oxidase. The enzyme retains its bioactivity in the rigid epoxy-graphite matrix. The electrocatalytic oxidation of hydrogen peroxide is enhanced by gold-palladium and it is used as the analytical signal (at 900 mV vs. Ag/AgCl in a pH 7.00 buffered solution with 0.1 M phosphate and 0.1 M KCl). This biosensor exhibits a linear response to glucose in the 0.01-2 mM range and produces steady-state signals within a few seconds (6 s for 95% signal). Simple polishing procedures can be used to generate a fresh bioactive transducer surface. © 1993.

Original language	English
Pages (from-to)	21-26
Journal	Analytica Chimica Acta
Volume	284
Issue number	1
DOIs	https://doi.org/10.1016/0003-2670(93)80003-4
Publication status	Published - 20 Dec 1993

Keywords

Amperometry
Biocomposites
Biosensors
Catalytic methods
Epoxy-graphite biocomposite
Glucose biosensor

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10.1016/0003-2670(93)80003-4

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title = "Amperometric glucose biosensor based on an electrocatalytically bulk-modified epoxy-graphite biocomposite",

abstract = "An inexpensive, robust, polishable and easily machinable amperometric glucose transducer was constructed. The biocomposite materials used were graphite, palladium-gold, non-conducting epoxy resin and glucose oxidase. The enzyme retains its bioactivity in the rigid epoxy-graphite matrix. The electrocatalytic oxidation of hydrogen peroxide is enhanced by gold-palladium and it is used as the analytical signal (at 900 mV vs. Ag/AgCl in a pH 7.00 buffered solution with 0.1 M phosphate and 0.1 M KCl). This biosensor exhibits a linear response to glucose in the 0.01-2 mM range and produces steady-state signals within a few seconds (6 s for 95% signal). Simple polishing procedures can be used to generate a fresh bioactive transducer surface. {\textcopyright} 1993.",

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T1 - Amperometric glucose biosensor based on an electrocatalytically bulk-modified epoxy-graphite biocomposite

AU - Céspedes, F.

AU - Martínez-Fàbregas, E.

AU - Alegret, S.

PY - 1993/12/20

Y1 - 1993/12/20

N2 - An inexpensive, robust, polishable and easily machinable amperometric glucose transducer was constructed. The biocomposite materials used were graphite, palladium-gold, non-conducting epoxy resin and glucose oxidase. The enzyme retains its bioactivity in the rigid epoxy-graphite matrix. The electrocatalytic oxidation of hydrogen peroxide is enhanced by gold-palladium and it is used as the analytical signal (at 900 mV vs. Ag/AgCl in a pH 7.00 buffered solution with 0.1 M phosphate and 0.1 M KCl). This biosensor exhibits a linear response to glucose in the 0.01-2 mM range and produces steady-state signals within a few seconds (6 s for 95% signal). Simple polishing procedures can be used to generate a fresh bioactive transducer surface. © 1993.

AB - An inexpensive, robust, polishable and easily machinable amperometric glucose transducer was constructed. The biocomposite materials used were graphite, palladium-gold, non-conducting epoxy resin and glucose oxidase. The enzyme retains its bioactivity in the rigid epoxy-graphite matrix. The electrocatalytic oxidation of hydrogen peroxide is enhanced by gold-palladium and it is used as the analytical signal (at 900 mV vs. Ag/AgCl in a pH 7.00 buffered solution with 0.1 M phosphate and 0.1 M KCl). This biosensor exhibits a linear response to glucose in the 0.01-2 mM range and produces steady-state signals within a few seconds (6 s for 95% signal). Simple polishing procedures can be used to generate a fresh bioactive transducer surface. © 1993.

KW - Amperometry

KW - Biocomposites

KW - Biosensors

KW - Catalytic methods

KW - Epoxy-graphite biocomposite

KW - Glucose biosensor

U2 - 10.1016/0003-2670(93)80003-4

DO - 10.1016/0003-2670(93)80003-4

M3 - Article

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JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

Amperometric glucose biosensor based on an electrocatalytically bulk-modified epoxy-graphite biocomposite

Abstract

Keywords

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