Amperometric determination of pesticides using a biosensor based on a polishable graphie-epoxy biocomposite

D. Martorell, F. Céspedes, E. Martínez-Fàbregas, S. Alegret

Research output: Contribution to journalArticleResearchpeer-review

99 Citations (Scopus)

Abstract

The determination of organophosphorus and carbamate pesticides was carried out using an amperometric transducer based on a robust, polishable and easily mechinable biocomposite. The biocomposite material contains graphite powder, a non-conducting epoxy resin and acetylcholinesterase. The enzyme retains its bioactivity in the rigid epoxy-graphite matric. Measurements were carried out with acetylhiocholine as a substrate. Thiocholine produced by enzymatic hydrolysis was oxidized electrochemically at 70 mV (vs. Ag/AgCl in pH 7.0 buffered solution with 0.1 M phosphate and 0.1 m KCl). The decrease rate of substrate steady-state current after the addition of pesticide was used for evaluation. The method of construction allows for the repetitive use of the electrode. Simple polishing procedures are used to regenerate the bioactive transducer surface. © 1994.
Original languageEnglish
Pages (from-to)343-348
JournalAnalytica Chimica Acta
Volume290
Issue number3
DOIs
Publication statusPublished - 30 May 1994

Keywords

  • Amperometry
  • Biosensors
  • Carbamate pesticides
  • Cholinesterase-based biosensor
  • Graphite-epoxy biocomposite
  • Organophosphorus pesticides
  • Pesticides

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