Amperometric determination of lysine using a lysine oxidase biosensor based on rigid-conducting composites

Javier Saurina, Santiago Hernández-Cassou, Salvador Alegret, Esteve Fàbregas

Research output: Contribution to journalArticleResearchpeer-review

35 Citations (Scopus)

Abstract

In this study, amperometric biosensors based on rigid conducting composites are developed for the determination of lysine. These lysine biosensors consist of chemically immobilized lysine oxidase membranes attached to either graphite-methacrylate or peroxidase-modified graphite- methacrylate electrodes. The enzymatic degradation of lysine releases hydrogen peroxide, which is the basis of the amperometric detection. The direct oxidation of hydrogen peroxide is monitored at + 1000 mV with a graphite-methacrylate electrode, while with the peroxidase-modified electrode reductive detection is performed. In addition, for the peroxidase-modified biocomposite electrode, both direct electron transfer and hydroquinone- mediated detection are studied. For the lysine biosensor based on the hydroquinone-mediated peroxidase biocomposite, the linear range is up to 1.6 x 10-4 M, the sensitivity 11300 μA/M, the repeatability 1.8%, the detection limit 8.2 x 10-7 M and the response time t(95%) is 42 s. The proposed biosensors are used to determine lysine in pharmaceutical samples. Results are consistent with those obtained with the standard method.
Original languageEnglish
Pages (from-to)211-220
JournalBiosensors and Bioelectronics
Volume14
Issue number2
DOIs
Publication statusPublished - 1 Feb 1999

Keywords

  • Amperometric biosensors
  • Graphite- methacrylate biocomposite
  • Hydroquinone- mediated detection
  • Lysine analysis
  • Lysine oxidase
  • Peroxidase-modified biocomposites
  • Rigid conducting composites

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