AMPA receptors are coupled to the nitric oxide/cyclic GMP pathway in cerebellar astroglial cells

M. A. Baltrons, A. Garcia

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27 Citations (Scopus)

Abstract

In cultured rat cerebellar astroglia kainate induces cGMP formation with low potency (EC50 310 μM). In the presence of cyclothiazide, a blocker of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor desensitization, the effect of kainate was potentiated and glutamate and AMPA elicited large increases (> 100-fold) in cGMP levels. The response to all three agonists was abolished by the nitric oxide synthase inhibitor N(G)-nitro-L-arginine and required extracellular calcium. Uptake of Co2+ was induced by AMPA in a limited population of astroglial cells and this effect was potentiated by cyclothiazide. These results indicate that calcium-permeable AMPA receptors mediate stimulation of nitric oxide formation in cerebellar astroglia. This effect may be relevant for glutamate-dependent synaptic plasticity processes in the cerebellum.
Original languageEnglish
Pages (from-to)2497-2501
JournalEuropean Journal of Neuroscience
Volume9
Issue number11
DOIs
Publication statusPublished - 1 Dec 1997

Keywords

  • Co uptake 2+
  • Cyclothiazide
  • Glutamate
  • Kainate
  • Rat

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