Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

Pau Vila; José L. Corchero; Antoni Benito; Antonio Villaverde

doi:10.1021/bp00030a010

Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

Pau Vila, José L. Corchero, Antoni Benito, Antonio Villaverde

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Abstract

The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)

Original language	English
Pages (from-to)	648-651
Journal	Biotechnology Progress
Volume	10
Issue number	6
DOIs	https://doi.org/10.1021/bp00030a010
Publication status	Published - 1 Jan 1994

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10.1021/bp00030a010

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title = "Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli",

abstract = "The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright {\textcopyright} 1994 American Institute of Chemical Engineers (AIChE)",

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N2 - The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)

AB - The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)

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DO - 10.1021/bp00030a010

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