Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

Pau Vila; José L. Corchero; Antoni Benito; Antonio Villaverde

doi:10.1021/bp00030a010

Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

Pau Vila, José L. Corchero, Antoni Benito, Antonio Villaverde

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Abstract

The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)

Original language	English
Pages (from-to)	648-651
Journal	Biotechnology Progress
Volume	10
Issue number	6
DOIs	https://doi.org/10.1021/bp00030a010
Publication status	Published - 1 Jan 1994

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10.1021/bp00030a010

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title = "Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli",

abstract = "The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright {\textcopyright} 1994 American Institute of Chemical Engineers (AIChE)",

author = "Pau Vila and Corchero, {Jos{\'e} L.} and Antoni Benito and Antonio Villaverde",

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T1 - Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

AU - Vila, Pau

AU - Corchero, José L.

AU - Benito, Antoni

AU - Villaverde, Antonio

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)

AB - The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)

U2 - 10.1021/bp00030a010

DO - 10.1021/bp00030a010

M3 - Article

VL - 10

SP - 648

EP - 651

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 6

ER -

Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

Abstract

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