Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

Pau Vila, José L. Corchero, Antoni Benito, Antonio Villaverde

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)
Original languageEnglish
Pages (from-to)648-651
JournalBiotechnology Progress
Volume10
Issue number6
DOIs
Publication statusPublished - 1 Jan 1994

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