Abstract
The effect of ammonium as a medium supplement on plasmid‐encoded recombinant β‐galactosidase synthesis was explored in Escherichia coli cells during aerobic growth in complex medium. After induction, only doses of ammonium chloride below 1 g/L are able to transiently enhance the yield. However, the presence of nontoxic ammonium chloride concentrations of up to 10 g/L results in lower values of β‐galactosidase in a concentration‐dependent fashion. A significant reduction in plasmid DNA content explains the decrease in the yield by a gene‐dosage‐involving mechanism. Copyright © 1994 American Institute of Chemical Engineers (AIChE)
Original language | English |
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Pages (from-to) | 648-651 |
Journal | Biotechnology Progress |
Volume | 10 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1 Jan 1994 |