Allosteric molecular sensing of anti-HIV antibodies by an immobilized engineered β-galactosidase

R. M. Ferraz, A. Aris, G. González, J. López-Santín, A. Villaverde, G. Álvaro

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

The main objective is to demonstrate the feasibility of qualitative and semi-quantitative determination of anti-HIV antibodies, based on the allosteric response of an immobilized engineered Escherichia coli β-galactosidase. The suitability of this enzyme and the conditions for homogeneous assay had been previously demonstrated. In the present work, the operational conditions for engineered E. coli β-galactosidase immobilization on activated agarose supports have been determined. A solid biocatalyst has been prepared and characterized in order to determine the activation degree of the immobilized enzyme in the presence of anti-HIV antibodies. The solid-phase biocatalyst has been shown to be able to modulate its activity and produce an unequivocal signal in presence of anti-HIV containing sera from different patients. Finally, the conceptual design of a possible assay based on the above methodology is proposed. © 2007 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)492-497
JournalEnzyme and Microbial Technology
Volume41
DOIs
Publication statusPublished - 3 Sep 2007

Keywords

  • Allosteric enzymes
  • Anti-HIV antibodies sensing
  • Immobilized engineered β-galactosidase

Fingerprint

Dive into the research topics of 'Allosteric molecular sensing of anti-HIV antibodies by an immobilized engineered β-galactosidase'. Together they form a unique fingerprint.

Cite this