Abstract
The main objective is to demonstrate the feasibility of qualitative and semi-quantitative determination of anti-HIV antibodies, based on the allosteric response of an immobilized engineered Escherichia coli β-galactosidase. The suitability of this enzyme and the conditions for homogeneous assay had been previously demonstrated. In the present work, the operational conditions for engineered E. coli β-galactosidase immobilization on activated agarose supports have been determined. A solid biocatalyst has been prepared and characterized in order to determine the activation degree of the immobilized enzyme in the presence of anti-HIV antibodies. The solid-phase biocatalyst has been shown to be able to modulate its activity and produce an unequivocal signal in presence of anti-HIV containing sera from different patients. Finally, the conceptual design of a possible assay based on the above methodology is proposed. © 2007 Elsevier Inc. All rights reserved.
Original language | English |
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Pages (from-to) | 492-497 |
Journal | Enzyme and Microbial Technology |
Volume | 41 |
DOIs | |
Publication status | Published - 3 Sep 2007 |
Keywords
- Allosteric enzymes
- Anti-HIV antibodies sensing
- Immobilized engineered β-galactosidase