Allergenicity and cross-reactivity of Senecio pollen: Identification of novel allergens using the immunoproteomics approach

O. Luengo, R. Mollá, C. Gámez, V. Cardona, E. López, B. Sastre, Y. Waisel, J. Belmonte, Á Cadahia, C. Lahoz, V. Del Pozo

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13 Citations (Scopus)

Abstract

Background: The genus Senecio is the largest genus of the family Asteraceae (Compositae). The allergenicity of Senecio has not been assessed previously. Objective: The aim of this study was to investigate the allergens of Senecio jacobea pollen and to determine their immunological characteristics and clinical relevance. Methods: Fifty patients with rhinoconjunctivitis and a positive skin prick test (SPT) to Senecio were recruited. The clinical relevance of this pollen was assessed by means of a nasal provocation test (NPT). Allergens were characterized by one-dimensional electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis and immunoblotting. Furthermore, characterization and identification of the allergens were performed by mass spectrometry (MS). In vitro inhibition tests were performed to evaluate cross-reactivity with other pollen. Results: Three predominant allergens, both in the intensity of reaction and the frequency of recognition by human-allergic sera, were 59 (60%), 42 (50%) and 31 kDa (50%). The two-dimensional analysis allowed the identification of several allergens. One spot around 42 kDa was identified as a protein homologous to pectate lyase and three other spots were homologous to malate dehydrogenase by MS. S. jacobea proteins showed cross-reactivity with other proteins of the Asteraceae family and also with Parietaria judaica. This was demonstrated by immunoblotting and ELISA inhibition studies. Conclusion: S. jacobea constitute a newly discovered allergenic source. It shows cross-reactivity with other members of the Asteraceae plant family as well as with P. judaica. © 2008 The Authors.
Original languageEnglish
Pages (from-to)1048-1060
JournalClinical and Experimental Allergy
Volume38
DOIs
Publication statusPublished - 1 Jun 2008

Keywords

  • Allergen
  • Asteraceae
  • Cross-reactivity
  • MALDI-TOF
  • Malate dehydrogenase
  • Nasal challenge
  • Pectate lyase
  • Pollen
  • Proteomics
  • Senecio

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