Aequorin-expressing yeast emits light under electric control

Cristina Vilanova; Ángeles Hueso; Carles Palanca; Guillem Marco; Miguel Pitarch; Eduardo Otero; Juny Crespo; Jerzy Szablowski; Sara Rivera; Laura Domínguez-Escribà; Emilio Navarro; Arnau Montagud; Pedro Fernández de Córdoba; Asier González; Joaquín Ariño; Andrés Moya; Javier Urchueguía; Manuel Porcar

doi:https://doi.org/10.1016/j.jbiotec.2011.01.005

Aequorin-expressing yeast emits light under electric control

Cristina Vilanova, Ángeles Hueso, Carles Palanca, Guillem Marco, Miguel Pitarch, Eduardo Otero, Juny Crespo, Jerzy Szablowski, Sara Rivera, Laura Domínguez-Escribà, Emilio Navarro, Arnau Montagud, Pedro Fernández de Córdoba, Asier González, Joaquín Ariño, Andrés Moya, Javier Urchueguía, Manuel Porcar

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. © 2011 Elsevier B.V.

Original language	English
Pages (from-to)	93-95
Journal	Journal of Biotechnology
Volume	152
DOIs	https://doi.org/10.1016/j.jbiotec.2011.01.005
Publication status	Published - 20 Mar 2011

Keywords

Aequorin
Bioelectronics
Bioluminescence
Synthetic biology
Yeast electro-stimulation

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https://doi.org/10.1016/j.jbiotec.2011.01.005

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Vilanova, C., Hueso, Á., Palanca, C., Marco, G., Pitarch, M., Otero, E., Crespo, J., Szablowski, J., Rivera, S., Domínguez-Escribà, L., Navarro, E., Montagud, A., de Córdoba, P. F., González, A., Ariño, J., Moya, A., Urchueguía, J., & Porcar, M. (2011). Aequorin-expressing yeast emits light under electric control. Journal of Biotechnology, 152, 93-95. https://doi.org/10.1016/j.jbiotec.2011.01.005

Vilanova, Cristina ; Hueso, Ángeles ; Palanca, Carles ; Marco, Guillem ; Pitarch, Miguel ; Otero, Eduardo ; Crespo, Juny ; Szablowski, Jerzy ; Rivera, Sara ; Domínguez-Escribà, Laura ; Navarro, Emilio ; Montagud, Arnau ; de Córdoba, Pedro Fernández ; González, Asier ; Ariño, Joaquín ; Moya, Andrés ; Urchueguía, Javier ; Porcar, Manuel. / Aequorin-expressing yeast emits light under electric control. In: Journal of Biotechnology. 2011 ; Vol. 152. pp. 93-95.

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title = "Aequorin-expressing yeast emits light under electric control",

abstract = "In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. {\textcopyright} 2011 Elsevier B.V.",

keywords = "Aequorin, Bioelectronics, Bioluminescence, Synthetic biology, Yeast electro-stimulation",

author = "Cristina Vilanova and {\'A}ngeles Hueso and Carles Palanca and Guillem Marco and Miguel Pitarch and Eduardo Otero and Juny Crespo and Jerzy Szablowski and Sara Rivera and Laura Dom{\'i}nguez-Escrib{\`a} and Emilio Navarro and Arnau Montagud and {de C{\'o}rdoba}, {Pedro Fern{\'a}ndez} and Asier Gonz{\'a}lez and Joaqu{\'i}n Ari{\~n}o and Andr{\'e}s Moya and Javier Urchuegu{\'i}a and Manuel Porcar",

year = "2011",

month = mar,

day = "20",

doi = "https://doi.org/10.1016/j.jbiotec.2011.01.005",

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Vilanova, C, Hueso, Á, Palanca, C, Marco, G, Pitarch, M, Otero, E, Crespo, J, Szablowski, J, Rivera, S, Domínguez-Escribà, L, Navarro, E, Montagud, A, de Córdoba, PF, González, A, Ariño, J, Moya, A, Urchueguía, J & Porcar, M 2011, 'Aequorin-expressing yeast emits light under electric control', Journal of Biotechnology, vol. 152, pp. 93-95. https://doi.org/10.1016/j.jbiotec.2011.01.005

Aequorin-expressing yeast emits light under electric control. / Vilanova, Cristina; Hueso, Ángeles; Palanca, Carles; Marco, Guillem; Pitarch, Miguel; Otero, Eduardo; Crespo, Juny; Szablowski, Jerzy; Rivera, Sara; Domínguez-Escribà, Laura; Navarro, Emilio; Montagud, Arnau; de Córdoba, Pedro Fernández; González, Asier; Ariño, Joaquín; Moya, Andrés; Urchueguía, Javier; Porcar, Manuel.

In: Journal of Biotechnology, Vol. 152, 20.03.2011, p. 93-95.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Aequorin-expressing yeast emits light under electric control

AU - Vilanova, Cristina

AU - Hueso, Ángeles

AU - Palanca, Carles

AU - Marco, Guillem

AU - Pitarch, Miguel

AU - Otero, Eduardo

AU - Crespo, Juny

AU - Szablowski, Jerzy

AU - Rivera, Sara

AU - Domínguez-Escribà, Laura

AU - Navarro, Emilio

AU - Montagud, Arnau

AU - de Córdoba, Pedro Fernández

AU - González, Asier

AU - Ariño, Joaquín

AU - Moya, Andrés

AU - Urchueguía, Javier

AU - Porcar, Manuel

PY - 2011/3/20

Y1 - 2011/3/20

N2 - In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. © 2011 Elsevier B.V.

AB - In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. © 2011 Elsevier B.V.

KW - Aequorin

KW - Bioelectronics

KW - Bioluminescence

KW - Synthetic biology

KW - Yeast electro-stimulation

U2 - https://doi.org/10.1016/j.jbiotec.2011.01.005

DO - https://doi.org/10.1016/j.jbiotec.2011.01.005

M3 - Article

VL - 152

SP - 93

EP - 95

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

ER -

Aequorin-expressing yeast emits light under electric control

Abstract

Keywords

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