TY - JOUR
T1 - Aequorin-expressing yeast emits light under electric control
AU - Vilanova, Cristina
AU - Hueso, Ángeles
AU - Palanca, Carles
AU - Marco, Guillem
AU - Pitarch, Miguel
AU - Otero, Eduardo
AU - Crespo, Juny
AU - Szablowski, Jerzy
AU - Rivera, Sara
AU - Domínguez-Escribà, Laura
AU - Navarro, Emilio
AU - Montagud, Arnau
AU - de Córdoba, Pedro Fernández
AU - González, Asier
AU - Ariño, Joaquín
AU - Moya, Andrés
AU - Urchueguía, Javier
AU - Porcar, Manuel
PY - 2011/3/20
Y1 - 2011/3/20
N2 - In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. © 2011 Elsevier B.V.
AB - In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5. V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. © 2011 Elsevier B.V.
KW - Aequorin
KW - Bioelectronics
KW - Bioluminescence
KW - Synthetic biology
KW - Yeast electro-stimulation
U2 - https://doi.org/10.1016/j.jbiotec.2011.01.005
DO - https://doi.org/10.1016/j.jbiotec.2011.01.005
M3 - Article
VL - 152
SP - 93
EP - 95
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
ER -