Advances in exosome analysis

Arnau Pallares-Rusiñol, Mireia Bernuz, Silio Lima Moura, Carolina Fernández-Senac, Rosanna Rossi, Mercè Martí, María Isabel Pividori

Research output: Chapter in BookChapterResearchpeer-review

1 Citation (Scopus)


There is growing demand for novel biomarkers that detect early stage disease as well as monitor clinical management and therapeutic strategies. Exosome analysis could provide the next advance in attaining that goal. Exosomes are membrane encapsulated biologic nanometric-sized particles of endocytic origin which are released by all cell types. Unfortunately, exosomes are exceptionally challenging to characterize with current technologies. Exosomes are between 30 and 200 nm in diameter, a size that makes them out of the sensitivity range to most cell-oriented sorting or analysis platforms, i.e., traditional flow cytometers. The most common methods for targeting exosomes to date typically involve purification followed by the characterization and the specific determination of their cargo. The whole procedure is time consuming, requiring thus skilled personnel as well as laboratory facilities and benchtop instrumentation. The most relevant methodology for exosome isolation, characterization and quantification is addressed in this chapter, including the most up-to-date approaches to explore the potential usefulness of exosomes as biomarkers in liquid biopsies and in advanced nanomedicine.

Original languageEnglish
Title of host publicationAdvances in Clinical Chemistry
EditorsGregory S. Makowski, Gregory S. Makowski
Number of pages49
Publication statusPublished - 1 Jan 2023

Publication series

NameAdvances in clinical chemistry
ISSN (Print)0065-2423


  • Exosomes
  • Flow cytometry
  • Immunomagnetic separation
  • Liquid biopsy
  • NTA
  • Nanovesicles
  • PCR
  • Solid-phase preconcentration
  • Liquid Biopsy
  • Humans
  • Exosomes/metabolism
  • Biomarkers/metabolism


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