Abstract
The current study was conducted to assess whether a single administration of 5-bromo-2'-deoxyuridine (BrdU) interferes with cell proliferation and leads to the activation of apoptotic cellular events in the prenatal cerebellum. BrdU effects across a wide range of doses (25-300 mu g/g b.w.) were analyzed using immunohistochemical and ultrastructural procedures. The pregnant rats were injected with BrdU at embryonic day 13, and their fetuses were sacrificed from 5 to 35 hr after exposure. The quantification of several parameters such as the density of mitotic figures, and BrdU and proliferating cell nuclear antigen (PCNA)-reactive cells showed that, in comparison with the saline injected rats, the administration of BrdU impairs the proliferative behavior of neuroepithelial cells. The above-mentioned parameters were significantly reduced in rats injected with 100 mu g/g b.w. of BrdU. The reduction was more evident using 200 mu g/g b.w. The most severe effects were found with 300 mu g/g b.w. of BrdU. The present findings also revealed that high doses of BrdU lead to the activation of apoptotic cellular events as evidenced by both terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and immunohistochemistry for active caspase-3. In comparison with saline rats, many apoptotic cells were found in rats injected with 100 mu g/g b.w. of BrdU. The number of dying cells increased with 200 mu g/g b.w. The most important number of apoptotic cells were observed in animals injected with 300 mu g/g b.w. of BrdU. Ultrastructural studies confirmed the presence of neuroblasts at different stages of apoptosis.
Original language | English |
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Article number | 529 |
Pages (from-to) | 1081-1096 |
Number of pages | 16 |
Journal | Journal of Comparative Neurology |
Volume | 529 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 Apr 2021 |
Keywords
- 5-bromo-2′-deoxyuridine
- RRID: AB_10013660
- RRID: AB_2313609
- RRID: AB_258588
- RRID: AB_476884
- RRID: AB_628110
- apoptosis
- cerebellar neuroepithelium
- embryonic life
- immunohistochemistry
- transmission electron microscopy
- NEURAL STEM
- 5-bromo-2 '-deoxyuridine
- ORIGIN
- BROMODEOXYURIDINE
- DNA
- 5-BROMODEOXYURIDINE
- NEUROGENESIS
- S-PHASE
- SENESCENCE
- CYCLE
- IMMUNOHISTOCHEMISTRY