Adenovirus-mediated introduction of DNA into pig sperm and offspring

Juan Enrique Rodriguez Gil, Maria Teresa Mogas Amoros, Teresa Josefa Rigau Mas, L. Farre, M. García-Rocha, M. Canal, A. M. Gomez-Foix

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    15 Citations (Scopus)

    Abstract

    The ability of adenoviral vectors to transfer DNA into boar spermatozoa and to offspring was tested. Exposure of spermatozoa to adenovirus bearing the E. coil lacZ gene resulted in the transfer of the gene to the head of the spermatozoa. Treatment did not affect either viability or acrosomal integrity of boar sperm. Of the 2-to 8-cell embryos obtained after in vitro fertilization with adenovirus-exposed sperm, 21.7% expressed the LacZ product. Four out of 56 piglets (about 7%) obtained after artificial insemination with adenovirus-exposed spermatozoa were positive in PCR analyses, even though none of the piglets showed the LacZ gene after southern blot analysis. RT-PCR analysis performed in tissues from two positive stillborn piglets showed the presence of the LacZ mRNA in all of the tissues tested. The offspring obtained after mating two positive animals did not show LacZ gene presence. Our results indicate that adenovirus could be a feasible mechanism for the delivery of DNA into spermatozoa, even though the transfer of the transgene may be limited to the first generation.
    Original languageEnglish
    Pages (from-to)149-158
    JournalMolecular Reproduction and Development
    Volume53
    Issue number2
    DOIs
    Publication statusPublished - 18 May 1999

    Keywords

    • Boar
    • DNA-transfer
    • Replication defective adenovirus
    • Spermatozoa

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