Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

B. A. Pereira; L. G.P. Rocha; M. C. Teles; W. E. Silva; J. A. Barbosa; S. S. Rabelo; A. S. Uchoa; J. E. Rodriguez-Gil; L. J. Pereira; M. G. Zangeronimo

doi:10.1590/1678-4162-10415

Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

B. A. Pereira, L. G.P. Rocha, M. C. Teles, W. E. Silva, J. A. Barbosa, S. S. Rabelo, A. S. Uchoa, J. E. Rodriguez-Gil, L. J. Pereira, M. G. Zangeronimo

Department of Animal Medicine and Surgery

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

© 2019 Universidade Federal de Minas Gerais. A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P < 0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P < 0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P < 0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P < 0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P < 0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

Original language	English
Pages (from-to)	489-499
Number of pages	11
Journal	Arquivo Brasileiro de Medicina Veterinaria e Zootecnia
Volume	71
Issue number	2
DOIs	https://doi.org/10.1590/1678-4162-10415
Publication status	Published - 1 Mar 2019

Keywords

Antioxidant
Boar semen storage
Metabolic activator
Methylxanthine
Polyphenol

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10.1590/1678-4162-10415

https://ddd.uab.cat/record/224128

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Pereira, B. A., Rocha, L. G. P., Teles, M. C., Silva, W. E., Barbosa, J. A., Rabelo, S. S., Uchoa, A. S., Rodriguez-Gil, J. E., Pereira, L. J., & Zangeronimo, M. G. (2019). Addition of chlorogenic acid and caffeine during the processing of cooled boar semen. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 71(2), 489-499. https://doi.org/10.1590/1678-4162-10415

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title = "Addition of chlorogenic acid and caffeine during the processing of cooled boar semen",

abstract = "{\textcopyright} 2019 Universidade Federal de Minas Gerais. A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P < 0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P < 0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P < 0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P < 0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P < 0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.",

keywords = "Antioxidant, Boar semen storage, Metabolic activator, Methylxanthine, Polyphenol",

author = "Pereira, {B. A.} and Rocha, {L. G.P.} and Teles, {M. C.} and Silva, {W. E.} and Barbosa, {J. A.} and Rabelo, {S. S.} and Uchoa, {A. S.} and Rodriguez-Gil, {J. E.} and Pereira, {L. J.} and Zangeronimo, {M. G.}",

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doi = "10.1590/1678-4162-10415",

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AU - Pereira, B. A.

AU - Rocha, L. G.P.

AU - Teles, M. C.

AU - Silva, W. E.

AU - Barbosa, J. A.

AU - Rabelo, S. S.

AU - Uchoa, A. S.

AU - Rodriguez-Gil, J. E.

AU - Pereira, L. J.

AU - Zangeronimo, M. G.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - © 2019 Universidade Federal de Minas Gerais. A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P < 0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P < 0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P < 0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P < 0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P < 0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

AB - © 2019 Universidade Federal de Minas Gerais. A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P < 0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P < 0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P < 0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P < 0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P < 0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

KW - Antioxidant

KW - Boar semen storage

KW - Metabolic activator

KW - Methylxanthine

KW - Polyphenol

UR - http://www.mendeley.com/research/addition-chlorogenic-acid-caffeine-during-processing-cooled-boar-semen

U2 - 10.1590/1678-4162-10415

DO - 10.1590/1678-4162-10415

M3 - Article

SN - 0102-0935

VL - 71

SP - 489

EP - 499

JO - Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

JF - Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

IS - 2

ER -

Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

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Keywords

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