Acinetobacter baumannii reca protein in repair of DNA damage, antimicrobial resistance, general stress response, and virulence

Jesús Aranda, Carlota Bardina, Alejandro Beceiro, Soraya Rumbo, Maria P. Cabral, Jordi Barbé, Germán Bou

Research output: Contribution to journalArticleResearchpeer-review

71 Citations (Scopus)

Abstract

RecA is the major enzyme involved in homologous recombination and plays a central role in SOS mutagenesis. In Acinetobacter spp., including Acinetobacter baumannii, a multidrug-resistant bacterium responsible for nosocomial infections worldwide, DNA repair responses differ in many ways from those of other bacterial species. In this work, the function of A. baumannii RecA was examined by constructing a recA mutant. Alteration of this single gene had a pleiotropic effect, showing the involvement of RecA in DNA damage repair and consequently in cellular protection against stresses induced by DNA damaging agents, several classes of antibiotics, and oxidative agents. In addition, the absence of RecA decreased survival in response to both heat shock and desiccation. Virulence assays in vitro (with macrophages) and in vivo (using a mouse model) similarly implicated RecA in the pathogenicity of A. baumannii. Thus, the data strongly suggest a protective role for RecA in the bacterium and indicate that inactivation of the protein can contribute to a combined therapeutic approach to controlling A. baumannii infections. © 2011, American Society for Microbiology.
Original languageEnglish
Pages (from-to)3740-3747
JournalJournal of Bacteriology
Volume193
Issue number15
DOIs
Publication statusPublished - 1 Aug 2011

Fingerprint Dive into the research topics of 'Acinetobacter baumannii reca protein in repair of DNA damage, antimicrobial resistance, general stress response, and virulence'. Together they form a unique fingerprint.

Cite this