Abscisic acid content of salt-stressed Phaseolus vulgaris L. Comparison of high-performance liquid chromatography, gas chromatography with electron-capture detection, enzyme-linked immunosorbent assay and radioimmunoassay

E. Montero, J. Sibole, C. Cabot, Ch Poschenrieder, J. Barceló

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17 Citations (Scopus)

Abstract

Sensitivity, reproducibility and cost effectiveness are important parameters to consider in analyses for abscisic acid (ABA). HPLC, GC with electron-capture detection (ECD), enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) were compared to determine the most appropriate method for the determination of ABA acid in salt-stressed bush bean plants. Determination of ABA by HPLC is better suited for samples with high ABA concentrations owing to the low selectivity of the UV detector. GC-ECD, ELISA and RIA are well suited for the assay of large numbers of samples and show good sensitivity for ABA. Analysis by RIA was the least costly method and required no sample purification process. © 1994.
Original languageEnglish
Pages (from-to)83-90
JournalJournal of Chromatography A
Volume658
Issue number1
DOIs
Publication statusPublished - 7 Jan 1994

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