A versatile perifusion system for the NMR spectroscopy of bovine retina. Assignment of resonances and effect of ischemia

Miquel E. Cabanas; Juli Alonso; Carles Arus; Mark E. Anderson; William M. Westler; John L. Markley

doi:10.1006/exer.1993.1175

A versatile perifusion system for the NMR spectroscopy of bovine retina. Assignment of resonances and effect of ischemia

Miquel E. Cabanas, Juli Alonso, Carles Arus, Mark E. Anderson, William M. Westler, John L. Markley

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25°C, as deduced from its 31P and 1H NMR spectra. The 31P NMR ex vivo spectrum of the retina has been characterized, and its major resonances have been assigned in tissue extracts. We have found that excised retinal tissue subjected to 2 hr of ischemia is capable, upon perifusion with oxygenated medium, of recovering its resting spectral pattern. Thus, this system offers a versatile model for studies of physiological events related with ischemia in a neural tissue, without associated systemic effects. © 1993 Academic Press, Inc.

Original language	English
Pages (from-to)	669-678
Journal	Experimental Eye Research
Volume	57
Issue number	6
DOIs	https://doi.org/10.1006/exer.1993.1175
Publication status	Published - 1 Jan 1993

Keywords

Bovine retina
Ischemia
Metabolism
Nuclear magnetic resonance spectroscopy
Perifusion system

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10.1006/exer.1993.1175

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@article{69db731b6f6d4d839c2e41c19e6058ac,

title = "A versatile perifusion system for the NMR spectroscopy of bovine retina. Assignment of resonances and effect of ischemia",

abstract = "A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25°C, as deduced from its 31P and 1H NMR spectra. The 31P NMR ex vivo spectrum of the retina has been characterized, and its major resonances have been assigned in tissue extracts. We have found that excised retinal tissue subjected to 2 hr of ischemia is capable, upon perifusion with oxygenated medium, of recovering its resting spectral pattern. Thus, this system offers a versatile model for studies of physiological events related with ischemia in a neural tissue, without associated systemic effects. {\textcopyright} 1993 Academic Press, Inc.",

keywords = "Bovine retina, Ischemia, Metabolism, Nuclear magnetic resonance spectroscopy, Perifusion system",

author = "Cabanas, {Miquel E.} and Juli Alonso and Carles Arus and Anderson, {Mark E.} and Westler, {William M.} and Markley, {John L.}",

year = "1993",

month = jan,

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doi = "10.1006/exer.1993.1175",

language = "English",

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A versatile perifusion system for the NMR spectroscopy of bovine retina. Assignment of resonances and effect of ischemia. / Cabanas, Miquel E.; Alonso, Juli; Arus, Carles; Anderson, Mark E.; Westler, William M.; Markley, John L.

In: Experimental Eye Research, Vol. 57, No. 6, 01.01.1993, p. 669-678.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - A versatile perifusion system for the NMR spectroscopy of bovine retina. Assignment of resonances and effect of ischemia

AU - Cabanas, Miquel E.

AU - Alonso, Juli

AU - Arus, Carles

AU - Anderson, Mark E.

AU - Westler, William M.

AU - Markley, John L.

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AB - A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25°C, as deduced from its 31P and 1H NMR spectra. The 31P NMR ex vivo spectrum of the retina has been characterized, and its major resonances have been assigned in tissue extracts. We have found that excised retinal tissue subjected to 2 hr of ischemia is capable, upon perifusion with oxygenated medium, of recovering its resting spectral pattern. Thus, this system offers a versatile model for studies of physiological events related with ischemia in a neural tissue, without associated systemic effects. © 1993 Academic Press, Inc.

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KW - Ischemia

KW - Metabolism

KW - Nuclear magnetic resonance spectroscopy

KW - Perifusion system

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