A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode

Abd Elgawad Radi; Xavier Muñoz-Berbel; Montserrat Cortina-Puig; Jean Louis Marty

doi:10.1002/elan.200904587

A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode

Abd Elgawad Radi, Xavier Muñoz-Berbel, Montserrat Cortina-Puig, Jean Louis Marty

Research output: Contribution to journal › Article › Research › peer-review

23 Citations (Scopus)

Abstract

A new convenient strategy to fabricate a third-generation hydrogen peroxide biosensor was described. The screen-printed carbon electrode (SPCE) was first modified with a layer of 4-nitrophenyl assembled from the 4-nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP-modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 μM to 50.0 μM, with a detection limit of 1.0 μM. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long-term stability. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	1624-1629
Journal	Electroanalysis
Volume	21
Issue number	14
DOIs	https://doi.org/10.1002/elan.200904587
Publication status	Published - 1 Jul 2009

Keywords

Biosensors
Diazonium salt
Direct electrochemistry
Enzymes
Horseradish peroxidase
Hydrogen peroxide
Screen-printed carbon electrode

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10.1002/elan.200904587

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title = "A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode",

abstract = "A new convenient strategy to fabricate a third-generation hydrogen peroxide biosensor was described. The screen-printed carbon electrode (SPCE) was first modified with a layer of 4-nitrophenyl assembled from the 4-nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP-modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 μM to 50.0 μM, with a detection limit of 1.0 μM. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long-term stability. {\textcopyright} 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",

keywords = "Biosensors, Diazonium salt, Direct electrochemistry, Enzymes, Horseradish peroxidase, Hydrogen peroxide, Screen-printed carbon electrode",

author = "Radi, {Abd Elgawad} and Xavier Mu{\~n}oz-Berbel and Montserrat Cortina-Puig and Marty, {Jean Louis}",

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doi = "10.1002/elan.200904587",

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A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode. / Radi, Abd Elgawad; Muñoz-Berbel, Xavier; Cortina-Puig, Montserrat et al.

In: Electroanalysis, Vol. 21, No. 14, 01.07.2009, p. 1624-1629.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode

AU - Radi, Abd Elgawad

AU - Muñoz-Berbel, Xavier

AU - Cortina-Puig, Montserrat

AU - Marty, Jean Louis

PY - 2009/7/1

Y1 - 2009/7/1

N2 - A new convenient strategy to fabricate a third-generation hydrogen peroxide biosensor was described. The screen-printed carbon electrode (SPCE) was first modified with a layer of 4-nitrophenyl assembled from the 4-nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP-modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 μM to 50.0 μM, with a detection limit of 1.0 μM. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long-term stability. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - A new convenient strategy to fabricate a third-generation hydrogen peroxide biosensor was described. The screen-printed carbon electrode (SPCE) was first modified with a layer of 4-nitrophenyl assembled from the 4-nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP-modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 μM to 50.0 μM, with a detection limit of 1.0 μM. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long-term stability. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Biosensors

KW - Diazonium salt

KW - Direct electrochemistry

KW - Enzymes

KW - Horseradish peroxidase

KW - Hydrogen peroxide

KW - Screen-printed carbon electrode

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DO - 10.1002/elan.200904587

M3 - Article

SN - 1040-0397

VL - 21

SP - 1624

EP - 1629

JO - Electroanalysis

JF - Electroanalysis

IS - 14

ER -

A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode

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Keywords

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