A third-generation hydrogen peroxide biosensor based on horseradish peroxidase covalently immobilized on electrografted organic film on screen-printed carbon electrode

Abd Elgawad Radi, Xavier Muñoz-Berbel, Montserrat Cortina-Puig, Jean Louis Marty

    Research output: Contribution to journalArticleResearchpeer-review

    22 Citations (Scopus)

    Abstract

    A new convenient strategy to fabricate a third-generation hydrogen peroxide biosensor was described. The screen-printed carbon electrode (SPCE) was first modified with a layer of 4-nitrophenyl assembled from the 4-nitroaniline diazonium salt synthesized in situ in acidic aqueous solution. Next, the nitro groups were converted to amines followed by crosslinking to the horseradish peroxidase (HRP) by glutaraldehyde. The redox chemistry of the active center of the HRP was observed and the HRP-modified electrode displayed electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2) without any mediators. H2O2 was determined in a linear range from 5.0 μM to 50.0 μM, with a detection limit of 1.0 μM. Furthermore, the biosensor exhibited fast amperometric response, good reproducibility and long-term stability. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Original languageEnglish
    Pages (from-to)1624-1629
    JournalElectroanalysis
    Volume21
    Issue number14
    DOIs
    Publication statusPublished - 1 Jul 2009

    Keywords

    • Biosensors
    • Diazonium salt
    • Direct electrochemistry
    • Enzymes
    • Horseradish peroxidase
    • Hydrogen peroxide
    • Screen-printed carbon electrode

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