A Simple Technique Based on a Single Optical Trap for the Determination of Bacterial Swimming Pattern

Ignacio A. Martínez, Susana Campoy*, Meritxell Tort, Montserrat Llagostera, Dmitri Petrov

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several Salmonella enterica serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the S: Typhimurium cheV mutant were characterized. © 2013 Martínez et al.
Original languageEnglish
Article numbere61630
JournalPLoS ONE
Volume8
Issue number4
DOIs
Publication statusPublished - 29 Apr 2013

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