A simple method for processing individual oocytes and embryos for electron microscopy

C. NOGUÉS; M. MARTÍ; M. BOADA; M. PONSÁ

doi:10.1111/j.1365-2818.1994.tb04324.x

A simple method for processing individual oocytes and embryos for electron microscopy

C. NOGUÉS, M. MARTÍ, M. BOADA, M. PONSÁ

Institute of Biotechnology and Biomedicine "Vicent Villar Palasi" (IBB)

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

A simple method for handling individual specimens that must be processed either for scanning or transmission electron microscopy studies is described. For scanning microscope processing, dehydration is carried out with samples enclosed in small cages made from TAAB capsules in which top and bottom are substituted by plankton nets, and for transmission electron microscopy, samples are pre‐embedded in agarose. This procedure significantly reduces mouth pipetting, dissecting microscope observations, is less labour intensive and, most importantly, reduces sample loss. 1994 Blackwell Science Ltd

Original language	English
Pages (from-to)	51-54
Journal	Journal of Microscopy
Volume	174
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2818.1994.tb04324.x
Publication status	Published - 1 Jan 1994

Keywords

embryo
individual specimens
Oocyte
SEM
TEM

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10.1111/j.1365-2818.1994.tb04324.x

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KW - SEM

KW - TEM

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A simple method for processing individual oocytes and embryos for electron microscopy

Abstract

Keywords

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