A simple and efficient method for the purification of an exopolysaccharide from Klebsiella sp. I-714

J. Farrés, G. Caminal, J. López-Santín

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2 Citations (Scopus)

Abstract

An easy separation of bacterial cells from the desired exopolysaccharide with an important reduction in protein content is achieved by adjusting the pH of the culture broth to 4 to 5 before autoclaving. There is a drastic reduction in the apparent viscosity, with formation of a cell aggregate which is easily sedimented. The resulting solution contains all the exopolysaccharide (the free, and the linked one), the amount of protein being reduced by 40%.
Original languageEnglish
Pages (from-to)375-380
JournalBiotechnology Techniques
Volume10
Issue number5
Publication statusPublished - 1 Dec 1996

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    Farrés, J., Caminal, G., & López-Santín, J. (1996). A simple and efficient method for the purification of an exopolysaccharide from Klebsiella sp. I-714. Biotechnology Techniques, 10(5), 375-380.