A quantitative real-time PCR method using an X-linked gene for sex typing in pigs

Maria Ballester; Anna Castelló; Yuliaxis Ramayo-Caldas; Josep M. Folch

doi:10.1007/s12033-012-9589-5

A quantitative real-time PCR method using an X-linked gene for sex typing in pigs

Maria Ballester, Anna Castelló, Yuliaxis Ramayo-Caldas, Josep M. Folch

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

At present, a wide range of molecular sex-typing protocols in wild and domestic animals are available. In pigs, most of these methods are based on PCR amplification of X-Y homologous genes followed by gel electrophoresis which is time-consuming and in some cases expensive. In this paper, we describe, for the first time, a SYBR green-based quantitative real-time PCR (qPCR) assay using an X-linked gene, the glycoprotein M6B, for genetic sexing of pigs. Taking into account the differences in the glycoprotein M6B gene copy number between genders, we determine the correct sex of 54 pig samples from either diaphragm or hair follicle from different breeds using the 2-ΔΔCT method for relative quantification. Our qPCR assay represents a quick, inexpensive, and reliable tool for sex determination in pigs. This new protocol could be easily adapted to other species in which the sex determination was required. © 2012 Springer Science+Business Media, LLC.

Original language	English
Pages (from-to)	493-496
Journal	Molecular Biotechnology
Volume	54
Issue number	2
DOIs	https://doi.org/10.1007/s12033-012-9589-5
Publication status	Published - 1 Jun 2013

Keywords

Copy number variation
Glycoprotein M6B
Pig sexing
Quantitative real-time PCR
SYBR green fluorophore
X-Linked gene

Access to Document

10.1007/s12033-012-9589-5

Cite this

@article{6c0fbb5522ef40d6b42c7903e41762d0,

title = "A quantitative real-time PCR method using an X-linked gene for sex typing in pigs",

abstract = "At present, a wide range of molecular sex-typing protocols in wild and domestic animals are available. In pigs, most of these methods are based on PCR amplification of X-Y homologous genes followed by gel electrophoresis which is time-consuming and in some cases expensive. In this paper, we describe, for the first time, a SYBR green-based quantitative real-time PCR (qPCR) assay using an X-linked gene, the glycoprotein M6B, for genetic sexing of pigs. Taking into account the differences in the glycoprotein M6B gene copy number between genders, we determine the correct sex of 54 pig samples from either diaphragm or hair follicle from different breeds using the 2-ΔΔCT method for relative quantification. Our qPCR assay represents a quick, inexpensive, and reliable tool for sex determination in pigs. This new protocol could be easily adapted to other species in which the sex determination was required. {\textcopyright} 2012 Springer Science+Business Media, LLC.",

keywords = "Copy number variation, Glycoprotein M6B, Pig sexing, Quantitative real-time PCR, SYBR green fluorophore, X-Linked gene",

author = "Maria Ballester and Anna Castell{\'o} and Yuliaxis Ramayo-Caldas and Folch, {Josep M.}",

year = "2013",

month = jun,

day = "1",

doi = "10.1007/s12033-012-9589-5",

language = "English",

volume = "54",

pages = "493--496",

journal = "Molecular Biotechnology",

issn = "1073-6085",

number = "2",

}

TY - JOUR

T1 - A quantitative real-time PCR method using an X-linked gene for sex typing in pigs

AU - Ballester, Maria

AU - Castelló, Anna

AU - Ramayo-Caldas, Yuliaxis

AU - Folch, Josep M.

PY - 2013/6/1

Y1 - 2013/6/1

N2 - At present, a wide range of molecular sex-typing protocols in wild and domestic animals are available. In pigs, most of these methods are based on PCR amplification of X-Y homologous genes followed by gel electrophoresis which is time-consuming and in some cases expensive. In this paper, we describe, for the first time, a SYBR green-based quantitative real-time PCR (qPCR) assay using an X-linked gene, the glycoprotein M6B, for genetic sexing of pigs. Taking into account the differences in the glycoprotein M6B gene copy number between genders, we determine the correct sex of 54 pig samples from either diaphragm or hair follicle from different breeds using the 2-ΔΔCT method for relative quantification. Our qPCR assay represents a quick, inexpensive, and reliable tool for sex determination in pigs. This new protocol could be easily adapted to other species in which the sex determination was required. © 2012 Springer Science+Business Media, LLC.

AB - At present, a wide range of molecular sex-typing protocols in wild and domestic animals are available. In pigs, most of these methods are based on PCR amplification of X-Y homologous genes followed by gel electrophoresis which is time-consuming and in some cases expensive. In this paper, we describe, for the first time, a SYBR green-based quantitative real-time PCR (qPCR) assay using an X-linked gene, the glycoprotein M6B, for genetic sexing of pigs. Taking into account the differences in the glycoprotein M6B gene copy number between genders, we determine the correct sex of 54 pig samples from either diaphragm or hair follicle from different breeds using the 2-ΔΔCT method for relative quantification. Our qPCR assay represents a quick, inexpensive, and reliable tool for sex determination in pigs. This new protocol could be easily adapted to other species in which the sex determination was required. © 2012 Springer Science+Business Media, LLC.

KW - Copy number variation

KW - Glycoprotein M6B

KW - Pig sexing

KW - Quantitative real-time PCR

KW - SYBR green fluorophore

KW - X-Linked gene

U2 - 10.1007/s12033-012-9589-5

DO - 10.1007/s12033-012-9589-5

M3 - Article

SN - 1073-6085

VL - 54

SP - 493

EP - 496

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 2

ER -

A quantitative real-time PCR method using an X-linked gene for sex typing in pigs

Abstract

Keywords

Access to Document

Fingerprint

Cite this