A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane

Arben Merkoçi, Susana Braga, Esteve Fàbregas, Salvador Alegret

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8 Citations (Scopus)

Abstract

A new potentiometric biosensor for d-amygdalin based on a consolidated biocomposite membrane has been designed. The manufacturing technique involved a simple compression of dry components presenting an ideal technology for the mass production of potentiometric biosensor devices. The effect of high pressures applied during the preparation of a d-amygdalin biosensor membrane was studied. The immobilisation of β-glucosidase was improved by increasing this pressure. The optimal biocomposite membrane composition was 100mg electroactive salts (AgI, Ag2S) and 10mg enzyme (50U). Characteristics such as linear range, response times and degree of enzyme immobilisation were studied for each of the pressures used in the production of the membranes. Copyright (C) 1999 Elsevier Science B.V.
Original languageEnglish
Pages (from-to)65-72
JournalAnalytica Chimica Acta
Volume391
Issue number1
DOIs
Publication statusPublished - 26 May 1999

Keywords

  • β-Glucosidase
  • Consolidated biocomposite
  • D-Amygdalin
  • Potentiometric biosensor

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