A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane

Arben Merkoçi; Susana Braga; Esteve Fàbregas; Salvador Alegret

doi:10.1016/S0003-2670(99)00201-9

A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane

Arben Merkoçi, Susana Braga, Esteve Fàbregas, Salvador Alegret

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

A new potentiometric biosensor for d-amygdalin based on a consolidated biocomposite membrane has been designed. The manufacturing technique involved a simple compression of dry components presenting an ideal technology for the mass production of potentiometric biosensor devices. The effect of high pressures applied during the preparation of a d-amygdalin biosensor membrane was studied. The immobilisation of β-glucosidase was improved by increasing this pressure. The optimal biocomposite membrane composition was 100mg electroactive salts (AgI, Ag2S) and 10mg enzyme (50U). Characteristics such as linear range, response times and degree of enzyme immobilisation were studied for each of the pressures used in the production of the membranes. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	65-72
Journal	Analytica Chimica Acta
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2670(99)00201-9
Publication status	Published - 26 May 1999

Keywords

β-Glucosidase
Consolidated biocomposite
D-Amygdalin
Potentiometric biosensor

Access to Document

10.1016/S0003-2670(99)00201-9

Cite this

@article{f1ad3933f4b64acf84382c19be47ad8c,

title = "A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane",

abstract = "A new potentiometric biosensor for d-amygdalin based on a consolidated biocomposite membrane has been designed. The manufacturing technique involved a simple compression of dry components presenting an ideal technology for the mass production of potentiometric biosensor devices. The effect of high pressures applied during the preparation of a d-amygdalin biosensor membrane was studied. The immobilisation of β-glucosidase was improved by increasing this pressure. The optimal biocomposite membrane composition was 100mg electroactive salts (AgI, Ag2S) and 10mg enzyme (50U). Characteristics such as linear range, response times and degree of enzyme immobilisation were studied for each of the pressures used in the production of the membranes. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "β-Glucosidase, Consolidated biocomposite, D-Amygdalin, Potentiometric biosensor",

author = "Arben Merko{\c c}i and Susana Braga and Esteve F{\`a}bregas and Salvador Alegret",

year = "1999",

month = may,

day = "26",

doi = "10.1016/S0003-2670(99)00201-9",

language = "English",

volume = "391",

pages = "65--72",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane

AU - Merkoçi, Arben

AU - Braga, Susana

AU - Fàbregas, Esteve

AU - Alegret, Salvador

PY - 1999/5/26

Y1 - 1999/5/26

N2 - A new potentiometric biosensor for d-amygdalin based on a consolidated biocomposite membrane has been designed. The manufacturing technique involved a simple compression of dry components presenting an ideal technology for the mass production of potentiometric biosensor devices. The effect of high pressures applied during the preparation of a d-amygdalin biosensor membrane was studied. The immobilisation of β-glucosidase was improved by increasing this pressure. The optimal biocomposite membrane composition was 100mg electroactive salts (AgI, Ag2S) and 10mg enzyme (50U). Characteristics such as linear range, response times and degree of enzyme immobilisation were studied for each of the pressures used in the production of the membranes. Copyright (C) 1999 Elsevier Science B.V.

AB - A new potentiometric biosensor for d-amygdalin based on a consolidated biocomposite membrane has been designed. The manufacturing technique involved a simple compression of dry components presenting an ideal technology for the mass production of potentiometric biosensor devices. The effect of high pressures applied during the preparation of a d-amygdalin biosensor membrane was studied. The immobilisation of β-glucosidase was improved by increasing this pressure. The optimal biocomposite membrane composition was 100mg electroactive salts (AgI, Ag2S) and 10mg enzyme (50U). Characteristics such as linear range, response times and degree of enzyme immobilisation were studied for each of the pressures used in the production of the membranes. Copyright (C) 1999 Elsevier Science B.V.

KW - β-Glucosidase

KW - Consolidated biocomposite

KW - D-Amygdalin

KW - Potentiometric biosensor

U2 - 10.1016/S0003-2670(99)00201-9

DO - 10.1016/S0003-2670(99)00201-9

M3 - Article

SN - 0003-2670

VL - 391

SP - 65

EP - 72

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

A potentiometric biosensor for D-amygdalin based on a consolidated biocomposite membrane

Abstract

Keywords

Access to Document

Fingerprint

Cite this